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Characterization of Arabidopsis thaliana SR protein genes: mutations, alternative splicing, and ESE selection

dc.contributor.advisorMount, Stephen Men_US
dc.contributor.authoredmonds, jason matthewen_US
dc.date.accessioned2007-09-28T14:57:39Z
dc.date.available2007-09-28T14:57:39Z
dc.date.issued2007-06-07en_US
dc.identifier.urihttp://hdl.handle.net/1903/7201
dc.description.abstractRNA processing in eukaryotes is a highly complex process requiring numerous steps and factors that can play roles in the regulation of functional protein production. SR proteins are a well-defined family of splicing factors identified by a conserved RNA Recognition Motif (RRM) and carboxyl-terminal arginine/serine (RS) repeats. SR proteins are known to bind to mRNA precursors via Exonic Splicing Enhancers, and to recruit U2AF and the U1 snRNP to promote splicing. I have identified mutations in five Arabidopsis thaliana SR protein genes that result in altered phenotypes. Two (scl28-1 and srp31-1) result in embryonic lethal phenotypes, while three others (sc35-1, sr45-1, and srp30-1) result in viable and fertile plants with a range of phenotypes. I have also found that mutations in individual SR protein genes can effect the ability of a specific sequence to act as an ESE and hence affect splicing efficiency. Because 16 of the 20 Arabidopsis thaliana SR proteins themselves are alternatively spliced, I have looked for cross regulation using RT-PCR analysis of isoform accumulation in alternatively spliced SR protein genes. I found that SR proteins do, in fact, regulate the alternative splicing of gene targets and do so in both a gene and a tissue specific manner. In order to begin to fully understand the relationship between individual SR proteins it is essential to know when and where they are expressed throughout development. I have studied the expression pattern of 16 of the 20 SR proteins in the roots of wild-type plants as well as sc35-1, srp30-1, and sr45-1 mutants. I have identified both spatial and temporal expression patterns for these 16 proteins relative to specific tissues that compose the root.en_US
dc.format.extent5869693 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleCharacterization of Arabidopsis thaliana SR protein genes: mutations, alternative splicing, and ESE selectionen_US
dc.typeDissertationen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.subject.pqcontrolledBiology, Geneticsen_US
dc.subject.pqcontrolledBiology, Molecularen_US
dc.subject.pquncontrolledSplicingen_US
dc.subject.pquncontrolledAlternative Splicingen_US
dc.subject.pquncontrolledSR proteinen_US
dc.subject.pquncontrolledRNAen_US
dc.subject.pquncontrolledmRNAen_US
dc.subject.pquncontrolledRNA processingen_US


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