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Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability

dc.contributor.authorJoardar, Vinita
dc.contributor.authorAbrams, Natalie F
dc.contributor.authorHostetler, Jessica
dc.contributor.authorPaukstelis, Paul J
dc.contributor.authorPakala, Suchitra
dc.contributor.authorPakala, Suman B
dc.contributor.authorZafar, Nikhat
dc.contributor.authorAbolude, Olukemi O
dc.contributor.authorPayne, Gary
dc.contributor.authorAndrianopoulos, Alex
dc.contributor.authorDenning, David W
dc.contributor.authorNierman, William C
dc.date.accessioned2021-09-28T18:26:20Z
dc.date.available2021-09-28T18:26:20Z
dc.date.issued2012-12-12
dc.identifierhttps://doi.org/10.13016/yxqh-slho
dc.identifier.citationJoardar, V., Abrams, N.F., Hostetler, J. et al. Sequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variability. BMC Genomics 13, 698 (2012).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28037
dc.description.abstractThe genera Aspergillus and Penicillium include some of the most beneficial as well as the most harmful fungal species such as the penicillin-producer Penicillium chrysogenum and the human pathogen Aspergillus fumigatus, respectively. Their mitochondrial genomic sequences may hold vital clues into the mechanisms of their evolution, population genetics, and biology, yet only a handful of these genomes have been fully sequenced and annotated. Here we report the complete sequence and annotation of the mitochondrial genomes of six Aspergillus and three Penicillium species: A. fumigatus, A. clavatus, A. oryzae, A. flavus, Neosartorya fischeri (A. fischerianus), A. terreus, P. chrysogenum, P. marneffei, and Talaromyces stipitatus (P. stipitatum). The accompanying comparative analysis of these and related publicly available mitochondrial genomes reveals wide variation in size (25–36 Kb) among these closely related fungi. The sources of genome expansion include group I introns and accessory genes encoding putative homing endonucleases, DNA and RNA polymerases (presumed to be of plasmid origin) and hypothetical proteins. The two smallest sequenced genomes (A. terreus and P. chrysogenum) do not contain introns in protein-coding genes, whereas the largest genome (T. stipitatus), contains a total of eleven introns. All of the sequenced genomes have a group I intron in the large ribosomal subunit RNA gene, suggesting that this intron is fixed in these species. Subsequent analysis of several A. fumigatus strains showed low intraspecies variation. This study also includes a phylogenetic analysis based on 14 concatenated core mitochondrial proteins. The phylogenetic tree has a different topology from published multilocus trees, highlighting the challenges still facing the Aspergillus systematics. The study expands the genomic resources available to fungal biologists by providing mitochondrial genomes with consistent annotations for future genetic, evolutionary and population studies. Despite the conservation of the core genes, the mitochondrial genomes of Aspergillus and Penicillium species examined here exhibit significant amount of interspecies variation. Most of this variation can be attributed to accessory genes and mobile introns, presumably acquired by horizontal gene transfer of mitochondrial plasmids and intron homing.en_US
dc.description.urihttps://doi.org/10.1186/1471-2164-13-698
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.subjectMitochondrial Genomeen_US
dc.subjectCore Geneen_US
dc.subjectAccessory Geneen_US
dc.subjectIntron Lossen_US
dc.subjectPenicillium Speciesen_US
dc.titleSequencing of mitochondrial genomes of nine Aspergillus and Penicillium species identifies mobile introns and accessory genes as main sources of genome size variabilityen_US
dc.typeArticleen_US
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtChemistry & Biochemistryen_us
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us


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