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Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time

dc.contributor.authorYang, Qianru
dc.contributor.authorDomesle, Kelly J
dc.contributor.authorWang, Fei
dc.contributor.authorGe, Beilei
dc.date.accessioned2021-07-28T14:54:44Z
dc.date.available2021-07-28T14:54:44Z
dc.date.issued2016-06-17
dc.identifierhttps://doi.org/10.13016/bur8-irrj
dc.identifier.citationYang, Q., Domesle, K.J., Wang, F. et al. Rapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-time. BMC Microbiol 16, 112 (2016).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27584
dc.description.abstractSalmonella is among the most significant pathogens causing food and feed safety concerns. This study examined the rapid detection of Salmonella in various types of food and feed samples by coupling loop-mediated isothermal amplification (LAMP) with a novel reporter, bioluminescent assay in real-time (BART). Performance of the LAMP-BART assay was compared to a conventional LAMP and the commercially available 3M Molecular Detection Assay (MDA) Salmonella. The LAMP-BART assay was 100 % specific among 178 strains (151 Salmonella and 27 non-Salmonella) tested. The detection limits were 36 cells per reaction in pure culture and 104 to 106 CFU per 25 g in spiked food and feed samples without enrichment, which were comparable to those of the conventional LAMP and 3M MDA Salmonella but 5–10 min faster. Ground turkey showed a strong inhibition on 3M MDA Salmonella, requiring at least 108 CFU per 25 g for detection. The correlation between Salmonella cell numbers and LAMP-BART signals was high (R 2 = 0.941–0.962), suggesting good quantification capability. After 24 h enrichment, all three assays accurately detected 1 to 3 CFU per 25 g of Salmonella among five types of food (cantaloupe, ground beef, ground turkey, shell eggs, and tomato) and three types of feed (cattle feed, chicken feed, and dry dog food) examined. However, 101 CFU per 25 g was required for cattle feed when tested by 3M MDA Salmonella. The Salmonella LAMP-BART assay was rapid, specific, sensitive, quantitative, and robust. Upon further validation, it may become a valuable tool for routine screening of Salmonella in various types of food and feed samples.en_US
dc.description.urihttps://doi.org/10.1186/s12866-016-0730-7
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.subjectLAMPen_US
dc.subjectBARTen_US
dc.subjectSalmonellaen_US
dc.subjectFooden_US
dc.subjectFeeden_US
dc.subjectDetectionen_US
dc.titleRapid detection of Salmonella in food and feed by coupling loop-mediated isothermal amplification with bioluminescent assay in real-timeen_US
dc.typeArticleen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtNutrition & Food Scienceen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us


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