Show simple item record

TRPV4, A CALCIUM-PERMEABLE CHANNEL, PLAYS A ROLE IN MATRIX STIFFNESS INDUCED MACROPHAGE POLARIZATION

dc.contributor.advisorRahaman, Shaik Ohidaren_US
dc.contributor.authorDutta, Bidishaen_US
dc.date.accessioned2020-10-08T05:30:34Z
dc.date.available2020-10-08T05:30:34Z
dc.date.issued2020en_US
dc.identifierhttps://doi.org/10.13016/n53i-ilms
dc.identifier.urihttp://hdl.handle.net/1903/26516
dc.description.abstractPhenotypic polarization of macrophages is deemed essential in innate immunity and various pathophysiological processes, but little is known about how mechanical forces like matrix stiffness regulate the polarization and the associated signaling mechanisms. Here we report that calcium ion channel Transient Receptor Potential Vanilloid 4 (TRPV4), a mechanosensitive receptor/channel, mediates the substrate stiffness-induced macrophage polarization. Using atomic force microscopy, we show that fibrosis-induced tissue stiffness is dependent on TRPV4. M1 macrophages were found to be the predominant macrophage subtype in stiffer tissues and loss of TRPV4 significantly decreased the level of M1 macrophages. These findings were further validated by our in vitro assays indicating that increase in substrate stiffness leads to an increased secretion of M1 proinflammatory mediators, which is further enhanced by the addition of soluble factors. Taken together, these findings provide new insights about the role of TRPV4 in matrix stiffness-induced macrophage polarization that can be explored in tissue engineering and in the development of targeted therapeutics.en_US
dc.language.isoenen_US
dc.titleTRPV4, A CALCIUM-PERMEABLE CHANNEL, PLAYS A ROLE IN MATRIX STIFFNESS INDUCED MACROPHAGE POLARIZATIONen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentNutritionen_US
dc.subject.pqcontrolledBiologyen_US
dc.subject.pqcontrolledBiomedical engineeringen_US
dc.subject.pqcontrolledCellular biologyen_US
dc.subject.pquncontrolledMacrophagesen_US
dc.subject.pquncontrolledPolarizationen_US
dc.subject.pquncontrolledSubstrate stiffnessen_US
dc.subject.pquncontrolledTRPV4en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record