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    Therapeutic Use of Bacteriophage and Antibiotic Formulations for the Treatment of Antibiotic Resistant Acinetobacter Baumannii

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    Date
    2020
    Author
    Acuña, Kierstin
    Barrie, Mariama
    Beaudry, Madeline
    Cooley, Rory
    Fields, Colin
    Grissom, Spencer
    Keepers, Zachery
    Lavrentieva, Anna
    Sutton, Hannah
    Walsh, Timothy
    Wittick, Natalie
    Advisor
    Knapstein, Kevin
    DRUM DOI
    https://doi.org/10.13016/xl10-dmlt
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    Abstract
    Widespread use of antibiotics has enriched global bacteria populations for strains possessing antibiotic resistance (AR) genes. Proliferation of AR genes and mechanisms have resulted in numerous multidrug resistant (MDR) infections for which there are no effective treatments. Acinetobacter baumannii is a major cause of hospital acquired (nosocomial) infections and is associated with outbreaks of MDR infections. Virulent bacteriophages (phages) present a way to remedy bacterial infections, while also having built-in mechanisms to circumvent resistance. This proposed study aims to develop a phage therapeutic targeting antibiotic resistant A. baumannii. The phages chosen for the final formulation exhibited high bactericidal activity and were able to infect several strains of A. baumannii from a provided library. Additionally, the phage-antibiotic synergy (PAS) effect was investigated in formulations with sub-lethal doses of ampicillin and chloramphenicol. The effectiveness of the phage therapeutic at different multiplicity of infections (MOI) and antibiotic concentrations were assessed relative to standard antibiotic doses. Well-plate studies suggest that higher MOI and antibiotic concentrations resulted in the greatest initial bactericidal effects, longest time to develop resistance, and lowest overall bacteria concentration. In future formulation studies, we would like to expand and optimize the current phage-antibiotic formulation and explore cocktail effects, whereby the formulation consists of a mixture of different phages that increases selective pressure.
    Notes
    Gemstone Team LYTIC
    URI
    http://hdl.handle.net/1903/25994
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    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility