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dc.contributor.advisorSriram, Ganeshen_US
dc.contributor.authorGraham, Trevoren_US
dc.date.accessioned2019-10-01T05:34:57Z
dc.date.available2019-10-01T05:34:57Z
dc.date.issued2019en_US
dc.identifierhttps://doi.org/10.13016/yosv-qwto
dc.identifier.urihttp://hdl.handle.net/1903/25103
dc.description.abstractUnderstanding cancer metabolism is critical to developing treatment strategies which selectively target malignant cells. Toward this objective, we apply isotope-assisted metabolic flux analysis to the investigation of prostate cancer, which kills over 28,000 men every year in the United States alone. We performed metabolic flux analysis (MFA) on immortal prostate cancer cell lines to determine the relative activity of metabolic pathways that constitute central carbon metabolism. We identified multiple deviations of the malignant phenotype from that of benign cells. We found that all cell lines exhibited a preference for the pentose phosphate pathway over glycolysis for glucose catabolism, with an average flux partition of 53% ± 25% in favor of the pentose phosphate pathway. We also identified a drop in TCA cycle flux from 33.5 ± 10.5 for LNCaP to 19.7 ± 7.8 for CSS90 cells, possibly indicating a preference for glutaminolysis and lipogenesis to fuel rapid proliferation.en_US
dc.language.isoenen_US
dc.titleISOTOPE-ASSISTED METABOLIC FLUX ANALYSIS IN THE INVESTIGATION OF PROSTATE CANCERen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentChemical Engineeringen_US
dc.subject.pqcontrolledChemical engineeringen_US
dc.subject.pquncontrolledIsotope Labeling Experimenten_US
dc.subject.pquncontrolledMetabolic Engineeringen_US
dc.subject.pquncontrolledMetabolic Flux Analysisen_US


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