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Long-term time-lapse live imaging reveals extensive cell migration during annelid regeneration

dc.contributor.authorZattara, Eduardo E.
dc.contributor.authorTurlington, Kate W.
dc.contributor.authorBely, Alexandra E.
dc.identifier.citationZattara, E.E., Turlington, K.W. & Bely, A.E. Long-term time-lapse live imaging reveals extensive cell migration during annelid regeneration. BMC Dev Biol 16, 6 (2016).en_US
dc.descriptionFunding for Open Access provided by the UMD Libraries Open Access Publishing Fund.en_US
dc.description.abstractBackground: Time-lapse imaging has proven highly valuable for studying development, yielding data of much finer resolution than traditional “still-shot” studies and allowing direct examination of tissue and cell dynamics. A major challenge for time-lapse imaging of animals is keeping specimens immobile yet healthy for extended periods of time. Although this is often feasible for embryos, the difficulty of immobilizing typically motile juvenile and adult stages remains a persistent obstacle to time-lapse imaging of post-embryonic development. Results: Here we describe a new method for long-duration time-lapse imaging of adults of the small freshwater annelid Pristina leidyi and use this method to investigate its regenerative processes. Specimens are immobilized with tetrodotoxin, resulting in irreversible paralysis yet apparently normal regeneration, and mounted in agarose surrounded by culture water or halocarbon oil, to prevent dehydration but allowing gas exchange. Using this method, worms can be imaged continuously and at high spatial-temporal resolution for up to 5 days, spanning the entire regeneration process. We performed a fine-scale analysis of regeneration growth rate and characterized cell migration dynamics during early regeneration. Our studies reveal the migration of several putative cell types, including one strongly resembling published descriptions of annelid neoblasts, a cell type suggested to be migratory based on “still-shot” studies and long hypothesized to be linked to regenerative success in annelids. Conclusions: Combining neurotoxin-based paralysis, live mounting techniques and a starvation-tolerant study system has allowed us to obtain the most extensive high-resolution longitudinal recordings of full anterior and posterior regeneration in an invertebrate, and to detect and characterize several cell types undergoing extensive migration during this process. We expect the tetrodotoxin paralysis and time-lapse imaging methods presented here to be broadly useful in studying other animals and of particular value for studying post-embryonic development.en_US
dc.publisherBioMed Centralen_US
dc.subjectAnnelid neoblasten_US
dc.subjectCell migrationen_US
dc.subjectDevelopmental dynamicsen_US
dc.subjectGrowth ratesen_US
dc.subjectIn-vivo studiesen_US
dc.subjectTime-lapse imagingen_US
dc.titleLong-term time-lapse live imaging reveals extensive cell migration during annelid regenerationen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us

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