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TIME SERIES METABOLIC PROFILING ANALYSIS OF THE SHORT TERM Arabidopsis thaliana RESPONSE TO ELEVATED CO2 USING GAS CHROMATOGRAPHY MASS SPECTROMETRY.

dc.contributor.advisorKlapa, Maria Ien_US
dc.contributor.authorKanani, Harin Haridasen_US
dc.date.accessioned2004-10-09T05:18:28Z
dc.date.available2004-10-09T05:18:28Z
dc.date.issued2004-08-30en_US
dc.identifier.urihttp://hdl.handle.net/1903/1880
dc.description.abstractMetabolic profiling has emerged as a high throughput technique for the quantitative analysis of the cellular physiological state at the metabolic level. It allows for the simultaneous relative quantification of hundreds of low molecular weight intra cellular metabolites. In this analysis, the polar metabolic profiles of A. thaliana liquid cultures (grown for 12 days, under light and 23°C) throughout 1-day treatment with 1% CO2, were measured using gas chromatography-mass spectrometry. Despite the advantages of time series analysis, this is the first plant metabolic profiling study of this type reported in the literature. The time series metabolic profiles were analyzed using multivariate statistical techniques. Data analysis revealed repression of photorespiration, repression of nitrogen assimilation and increase in structural carbohydrates. It is for the first time that the latter phenomenon is observed as a result of elevated CO2 in the plant environment.en_US
dc.format.extent3176812 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleTIME SERIES METABOLIC PROFILING ANALYSIS OF THE SHORT TERM Arabidopsis thaliana RESPONSE TO ELEVATED CO2 USING GAS CHROMATOGRAPHY MASS SPECTROMETRY.en_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentChemical Engineeringen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pqcontrolledBiology, Plant Physiologyen_US
dc.subject.pquncontrolledMetabolic Profilingen_US
dc.subject.pquncontrolledMetabolomicsen_US
dc.subject.pquncontrolledArabidopsis thalianaen_US
dc.subject.pquncontrolledElevated CO2en_US
dc.subject.pquncontrolledMultivariate Statistical Analysisen_US
dc.subject.pquncontrolledSystems Biologyen_US


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