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dc.contributor.advisorFisher, John Pen_US
dc.contributor.authorBall, Owen Matthewen_US
dc.date.accessioned2016-02-09T06:32:01Z
dc.date.available2016-02-09T06:32:01Z
dc.date.issued2015en_US
dc.identifierhttps://doi.org/10.13016/M24H83
dc.identifier.urihttp://hdl.handle.net/1903/17345
dc.description.abstractThere is a significant clinical need for engineered bone graft substitutes that can quickly, effectively, and safely repair segmental bone defects. One emerging field of interest involves the growth of engineered bone tissue in vitro within bioreactors, the most promising of which, are perfusion bioreactors. Utilizing a tubular perfusion system bioreactor, which allows media to perfuse freely around alginate scaffolds laden with human mesenchymal stem cells, large-scale bone constructs can be created by simply aggregating these beads together in the desired shape. However, these engineered constructs lack inherent vasculature and quickly develop a necrotic core, where no nutrient exchange occurs. Through the use of 3D printed vascular structures, used in conjunction with a TPS bioreactor, cell viability after just one day of aggregation was found to increase by as much as 50 percent in the core of these constructs, with in silico modeling predicting construct viability at steady state.en_US
dc.language.isoenen_US
dc.titleEFFECTS OF 3D PRINTED VASCULAR NETWORKS ON HUMAN MESENCHYMAL STEM CELL VIABILITY IN LARGE BONE TISSUE CONSTRUCTSen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentBioengineeringen_US
dc.subject.pqcontrolledBiomedical engineeringen_US
dc.subject.pquncontrolled3D printingen_US
dc.subject.pquncontrolledBioreactoren_US
dc.subject.pquncontrolledBoneen_US
dc.subject.pquncontrolledComputational Modelingen_US
dc.subject.pquncontrolledhMSCsen_US
dc.subject.pquncontrolledVasculatureen_US


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