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Back to Earth: Molecular Approaches to Microbial Ecology Must Consider Soil Morphology and Physicochemical Properties

dc.contributor.advisorYarwood, Stephanie Aen_US
dc.contributor.authorDlott, Gladeen_US
dc.date.accessioned2015-07-17T05:32:02Z
dc.date.available2015-07-17T05:32:02Z
dc.date.issued2015en_US
dc.identifierhttps://doi.org/10.13016/M2C91J
dc.identifier.urihttp://hdl.handle.net/1903/16782
dc.description.abstractThis project studied the influence of different long-term agricultural management regimes on soil microbial communities, and compared survival strategies of individual prokaryotic OTUs in diverse soils subjected to long-term incubation. Together these would show whether alterations to microbial communities affect rates of soil carbon cycling. Agricultural soils were sampled at arbitrary depths above and below the plow layer, and relative abundances of microbes were measured using high-throughput sequencing. `Activity' (rRNA:rDNA) ratios were calculated for individual OTUs identified by high-throughput sequencing of tropical rainforest and temperate cornfield soils after incubation for one year with differing water and carbon availabilities. It was found that depth controls microbial communities to a greater degree than agricultural management, and that the characterization of microbial trophic strategies might be complicated by the often-ignored DNA preservation potential of soil. The study highlights the need for holistic approaches to testing hypotheses in modern microbial ecology.en_US
dc.language.isoenen_US
dc.titleBack to Earth: Molecular Approaches to Microbial Ecology Must Consider Soil Morphology and Physicochemical Propertiesen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentEnvironmental Science and Technologyen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pqcontrolledSoil sciencesen_US
dc.subject.pqcontrolledEcologyen_US
dc.subject.pquncontrolledAgricultureen_US
dc.subject.pquncontrolledCommunityen_US
dc.subject.pquncontrolledDNA Preservationen_US
dc.subject.pquncontrolledMicrobial Ecologyen_US
dc.subject.pquncontrolledSoil Depthen_US
dc.subject.pquncontrolledSpecific Activityen_US


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