Skip to content
University of Maryland LibrariesDigital Repository at the University of Maryland
    • Login
    View Item 
    •   DRUM
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • View Item
    •   DRUM
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Mesenchymal Stem Cell Enrichment and Differentiation through Functionalized Biomaterials

    Thumbnail
    View/Open
    Ferlin_umd_0117E_16208.pdf (7.597Mb)
    No. of downloads: 250

    Date
    2015
    Author
    Ferlin, Kimberly Marie
    Advisor
    Fisher, John P
    DRUM DOI
    https://doi.org/10.13016/M2W92K
    Metadata
    Show full item record
    Abstract
    The use of mesenchymal stem cells (MSCs) for tissue engineering and cell-based therapies has great potential. MSCs are an adult stem cell population capable of undergoing multilineage differentiation into several key tissue types including bone, fat, and cartilage. However, despite key research and clinical advances, these therapies are still largely in the developmental phases. MSCs are typically isolated from the bone marrow using a multi-step process involving density centrifugation and adherence of the mononuclear fraction of cells to tissue culture polystyrene (TCPS). The majority of MSC-based approaches require in vitro cell expansion in monolayer to produce the cell numbers necessary for subsequent implantation. Recently, it has been suggested that expansion may cause significant changes to the MSC phenotype. In an effort to simplify the process of MSC isolation and use for such applications, our goal was to develop a single-step 3D culture system for the capture, culture, and differentiation of MSCs. To this end, we focused on the adhesion of MSCs to an underlying substrate, specifically how adhesion could facilitate one-step isolation. We showed that there are distinct changes in MSC adhesion during differentiation that can be used to separate populations of differentiating cells to decrease the heterogeneity of the cell population for implantation. By tethering proteins typically found in the MSC extracellular matrix onto the surface of polymer scaffolds, we were able to increase the specific adhesion of MSCs over TCPS, the current gold standard. Additionally, surface functionalization could be used as a method to drive rapid and directed differentiation of MSCs. Upon exposure to the heterogeneous population of the bone marrow, cells captured on the functionalized material surface had a similar phenotypic signature to MSC controls. Using 3D printing technology, our polymer scaffolds were translated into a highly controlled 3D environment that supports MSC adhesion, proliferation, and differentiation. The techniques presented here represent the key criteria for the development of a one-step culture system for MSC isolation and subsequent implantation. This work highlights the feasibility of functionalized biomaterials as a means to simplify the current use of MSCs for regenerative medicine.
    URI
    http://hdl.handle.net/1903/16778
    Collections
    • Fischell Department of Bioengineering Theses and Dissertations
    • UMD Theses and Dissertations

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility
     

     

    Browse

    All of DRUMCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    Pages
    About DRUMAbout Download Statistics

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility