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dc.contributor.advisorYonkos, Lance
dc.contributor.authorKim, Christine
dc.contributor.authorLeasca, Angela
dc.contributor.authorLiu, Winston
dc.contributor.authorPatel, Shivani
dc.contributor.authorPoulsen, Laura
dc.contributor.authorShah, Shefali
dc.contributor.authorThrowe, Taylor
dc.contributor.authorTripu, Renuka
dc.date.accessioned2015-07-14T15:20:00Z
dc.date.available2015-07-14T15:20:00Z
dc.date.issued2015
dc.identifierdoi:10.13016/M25S7S
dc.identifier.urihttp://hdl.handle.net/1903/16768
dc.description.abstractIntersex in largemouth bass (Micropterus salmoides) has been correlated with regional anthropogenic activity, but has not been causally linked to environmental factors. Four groups of hatchery-reared largemouth bass (LMB) and fathead minnows (FHM) of varying ages and sex were exposed to aqueous poultry litter mixtures, 17β- estradiol (E2), and controls. Water samples were analyzed for estrogens through liquid chromatography tandem mass spectrometry and estrogenicity through the bioluminescent yeast estrogen screen assay. Fish plasma was analyzed for the egg yolk protein vitellogenin (Vtg) using enzyme–linked immunosorbent assay and gonad tissue was examined histologically for enumeration of testicular oocytes (TO). Water chemistry revealed typical E2 conversion to Estrone with subsequent decay over the exposure periods. A modest prevalence of TO (9.4%) was detected with no apparent treatment effect. While significant Vtg induction was found in E2 exposed FHM, minimal Vtg induction was found in male LMB. Despite field findings of intersex in male LMB, this species may be poorly suited for laboratory investigations into endocrine disruption.en_US
dc.language.isoen_USen_US
dc.subjectEndocrine disruptionen_US
dc.subjectIntersexen_US
dc.subjectTesticular oocytesen_US
dc.subjectVitellogeninen_US
dc.subjectLargemouth bassen_US
dc.subjectPoultry litteren_US
dc.subjectFathead minnowen_US
dc.subjectEstradiolen_US
dc.subjectGemstone Team BASSen_US
dc.titleAppropriateness of Largemouth Bass as a Model Species for Detection of Endocrine Dissruptionen_US
dc.typeThesisen_US
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtGemstone Program, University of Maryland (College Park, Md)


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