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Development of Techniques for Plasma Membrane and Cell Surface Enrichment

dc.contributor.advisorFenselau, Catherineen_US
dc.contributor.authorRose, Rebecca Leighen_US
dc.date.accessioned2015-06-25T05:31:07Z
dc.date.available2015-06-25T05:31:07Z
dc.date.issued2014en_US
dc.identifierhttps://doi.org/10.13016/M2B90V
dc.identifier.urihttp://hdl.handle.net/1903/16400
dc.description.abstractThe goal of this work is to develop and compare two methods of cell surface protein enrichment. The plasma membrane and cell surface proteomes are of great interest for the development of drug targets and increasing our understanding of communication pathways, especially in disease states. Here we evaluate the enrichment of the plasma membrane proteome utilizing the nanowire pellicle technique. The pellicle is constructed with iron silicate or silica core nanowires to observe the effects of wire density upon enrichment success. Comparison of these two wire pellicles reveals a two-fold enrichment of transmembrane proteins, over a multiple myeloma whole cell lysate, in both samples, with a slightly higher trend in the iron silicate nanowire pellicle. A covalent tagging method was also undertaken to assess its potential application to the study of myeloid-derived suppressor (MDSC) cell surfaces. This method couples glycan oxidation with biotinylation and enrichment on immobilized streptavidin to isolate surface glycoproteins. Application of this method to myeloid-derived suppressor cells yielded cell surface enrichment values ranging from approximately 50-60%. Furthermore, several protein groups of interest were identified for further assessment.en_US
dc.language.isoenen_US
dc.titleDevelopment of Techniques for Plasma Membrane and Cell Surface Enrichmenten_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentBiochemistryen_US
dc.subject.pqcontrolledBiochemistryen_US
dc.subject.pqcontrolledCellular biologyen_US
dc.subject.pqcontrolledAnalytical chemistryen_US
dc.subject.pquncontrolledCanceren_US
dc.subject.pquncontrolledCell surfaceen_US
dc.subject.pquncontrolledMethods developmenten_US
dc.subject.pquncontrolledNanowireen_US
dc.subject.pquncontrolledPellicleen_US
dc.subject.pquncontrolledPlasma membraneen_US


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