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A whole-genome assembly of the domestic cow, Bos taurus

dc.contributor.authorZimin, Aleksey V
dc.contributor.authorDelcher, Arthur L
dc.contributor.authorFlorea, Liliana
dc.contributor.authorKelley, David R
dc.contributor.authorSchatz, Michael C
dc.contributor.authorPuiu, Daniela
dc.contributor.authorHanrahan, Finnian
dc.contributor.authorPertea, Geo
dc.contributor.authorVan Tassell, Curtis P
dc.contributor.authorSonstegard, Tad S
dc.contributor.authorMarcais, Guillaume
dc.contributor.authorRoberts, Michael
dc.contributor.authorSubramanian, Poorani
dc.contributor.authorYorke, James A
dc.contributor.authorSalzberg, Steven L
dc.identifier.citationZimin, A. V., Marcais, G., Roberts, M., Subramanian, P., Yorke, J. A., Delcher, A. L., Florea, L., ... Sonstegard, T. S. (April 24, 2009). A whole-genome assembly of the domestic cow, Bos taurus. Genome Biology, 10, 4.en_US
dc.description.abstractBackground: The genome of the domestic cow, Bos taurus, was sequenced using a mixture of hierarchical and whole-genome shotgun sequencing methods. Results: We have assembled the 35 million sequence reads and applied a variety of assembly improvement techniques, creating an assembly of 2.86 billion base pairs that has multiple improvements over previous assemblies: it is more complete, covering more of the genome; thousands of gaps have been closed; many erroneous inversions, deletions, and translocations have been corrected; and thousands of single-nucleotide errors have been corrected. Our evaluation using independent metrics demonstrates that the resulting assembly is substantially more accurate and complete than alternative versions. Conclusions: By using independent mapping data and conserved synteny between the cow and human genomes, we were able to construct an assembly with excellent large-scale contiguity in which a large majority (approximately 91%) of the genome has been placed onto the 30 B. taurus chromosomes. We constructed a new cow-human synteny map that expands upon previous maps. We also identified for the first time a portion of the B. taurus Y chromosome.en_US
dc.subjectgenome assemblyen_US
dc.subjectBos taurusen_US
dc.titleA whole-genome assembly of the domestic cow, Bos taurusen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us

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