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JUNCTION PROBES - SEQUENCE SPECIFIC DETECTION OF NUCLEIC ACIDS VIA TEMPLATE ENHANCED HYBRIDIZATION PROCESSES

dc.contributor.advisorSintim, Herman Oen_US
dc.contributor.authorYan, Leien_US
dc.date.accessioned2011-07-08T05:36:35Z
dc.date.available2011-07-08T05:36:35Z
dc.date.issued2011en_US
dc.identifier.urihttp://hdl.handle.net/1903/11792
dc.description.abstractAs new disease biomarkers such as cancer-linked microRNAs are discovered, the need for new strategies for the detection of these disease biomarkers at isothermal conditions will increase. The junction probe (JP) technology is a restriction endonuclease-based nucleic acids detection platform that achieves isothermal amplified sensing and does not require the presence of an endonuclease recognition sequence in the target analyte. The first generation junction probe platform however suffered from long assay time (several hours). We hypothesized that the slow catalysis in the first-generation JP platform was due to an inhibition cycle. Consequently, a second generation JP platform, which is modified with phosphorothioate moieties in order to suppress the inhibition cycle, was developed. The second-generation JP platform is significantly superior to the first-generation platform and we demonstrated the potential of second-generation JP by detecting microRNA and bacterial ribosomal RNA. Importantly, second-generation JP could detect RNA in crude bacterial cell lysates without extensive sample preparation.en_US
dc.titleJUNCTION PROBES - SEQUENCE SPECIFIC DETECTION OF NUCLEIC ACIDS VIA TEMPLATE ENHANCED HYBRIDIZATION PROCESSESen_US
dc.typeDissertationen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentChemistryen_US
dc.subject.pqcontrolledBiochemistryen_US


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