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Effects of 17-α-ethynylestradiol on Hybrid Striped Bass Sperm

dc.contributor.advisorWoods, L. Curry
dc.contributor.authorDouglas, Matthew
dc.contributor.authorDubik, Bradford
dc.contributor.authorHaq, Sarah
dc.contributor.authorJiang, Yike
dc.contributor.authorLe, TuQuynh
dc.contributor.authorLemanski, Natalie
dc.contributor.authorLinkins, Samantha
dc.contributor.authorMurphy, Kaitlyn
dc.contributor.authorMcFarland, Genna
dc.contributor.authorPeitzmeier, Sarah
dc.contributor.authorRahman, Aziz
dc.contributor.authorSalins, Mickey
dc.contributor.authorTheisen, Catherine
dc.descriptionGemstone Team FISH
dc.description.abstractOne of the most potent EDCs in the environment is 17-ethynylestradiol (EE2), the hormone in most birth control pills. EE2 is released into the ecosystem through human wastewater, affecting the environment and its inhabitants. Fish both live and reproduce in these affected ecosystems, which may make them particularly susceptible to the effects of EE2. This study investigates the impacts on reproductive efficacy of acute, direct exposure of male hybrid striped bass sperm cells to EE2. In the study, reproductive efficacy is measured by two endpoints: genetic integrity of sperm DNA and sperm cell viability. Genetic integrity and cell viability were assessed by the comet assay and SYBR-14/Propidium Iodide stains, respectively. The results concerning genetic integrity were not statistically significant, but the results of the sperm viability assay suggest that acute direct exposure to EE2 does not cause significant death within a population of sperm.en_US
dc.subjectEndocrine disrupting compoundsen_US
dc.subjectGemstone Team FISH
dc.titleEffects of 17-α-ethynylestradiol on Hybrid Striped Bass Spermen_US
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtGemstone Program, University of Maryland (College Park, Md)

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