College of Agriculture & Natural Resources

Permanent URI for this communityhttp://hdl.handle.net/1903/1598

The collections in this community comprise faculty research works, as well as graduate theses and dissertations.

Browse

Subject: search.filters.subject.lipid oxidation

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Dietary Macronutrient Composition Differentially Modulates the Remodeling of Mitochondrial Oxidative Metabolism during NAFLD
    (MDPI, 2021-04-26) Kattapuram, Nathan; Zhang, Christine; Muyyarikkandy, Muhammed S.; Surugihalli, Chaitra; Muralidaran, Vaishna; Gregory, Tabitha; Sunny, Nishanth E.
    Diets rich in fats and carbohydrates aggravate non-alcoholic fatty liver disease (NAFLD), of which mitochondrial dysfunction is a central feature. It is not clear whether a high-carbohydrate driven ‘lipogenic’ diet differentially affects mitochondrial oxidative remodeling compared to a high-fat driven ‘oxidative’ environment. We hypothesized that the high-fat driven ‘oxidative’ environment will chronically sustain mitochondrial oxidative function, hastening metabolic dysfunction during NAFLD. Mice (C57BL/6NJ) were reared on a low-fat (LF; 10% fat calories), high-fat (HF; 60% fat calories), or high-fructose/high-fat (HFr/HF; 25% fat and 34.9% fructose calories) diet for 10 weeks. De novo lipogenesis was determined by measuring the incorporation of deuterium from D2O into newly synthesized liver lipids using nuclear magnetic resonance (NMR) spectroscopy. Hepatic mitochondrial metabolism was profiled under fed and fasted states by the incubation of isolated mitochondria with [13C3]pyruvate, targeted metabolomics of tricarboxylic acid (TCA) cycle intermediates, estimates of oxidative phosphorylation (OXPHOS), and hepatic gene and protein expression. De novo lipogenesis was higher in the HFr/HF mice compared to their HF counterparts. Contrary to our expectations, hepatic oxidative function after fasting was induced in the HFr/HF group. This differential induction of mitochondrial oxidative function by the high fructose-driven ‘lipogenic’ environment could influence the progressive severity of hepatic insulin resistance.
  • Thumbnail Image
    Item
    Inhibitory Effect of Selected Spice and Fruit Seed Extracts on Lipid Oxidation in Fish Oil and Their Radical Scavenging and Antimicrobial Properties
    (2006-09-27) Luther, Marla West; Yu, Liangli; Food Science; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Ethanol extracts of cumun-3 parsley, black currant, green river parsley, Chardonnay grape, Pinot noir grape and black raspberry seed flours and cranberry seed meal were evaluated for their capacity to suppress lipid oxidation, preserve fatty acids, inhibit microbial growth, and scavenge DPPH and peroxyl radicals (ORAC), and total phenolic content (TPC). All tested extracts suppressed lipid oxidation in fish oil. At a concentration range of 2.6-5.3 mg flour or meal equivalents/mL all extracts exhibited antibacterial activity against Escherichia coli and Listeria monocytogenes, except cumun-3 and green river parsley against L. monocytogenes. All tested seed flour and meal extracts exhibited DPPH radical quenching activity. Chardonnay exhibited the strongest ORAC of 663 µmol TE/g and highest TPC of 99 mg GAE/g seed flour. The data from this study suggest the potential of developing natural food preservatives from these seed flours and meal for improving food stability, quality, safety and consumer acceptance.