College of Agriculture & Natural Resources

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Subject: search.filters.subject.Synbiotic

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    Synbiotic-like effect of linoleic acid overproducing Lactobacillus casei with berry phenolic extracts against pathogenesis of enterohemorrhagic Escherichia coli
    (Springer Nature, 2019-07-27) Tabashsum, Zajeba; Peng, Mengfei; Bernhardt, Cassendra; Patel, Puja; Carrion, Michael; Biswas, Debabrata
    Majority of enteric infections are foodborne and antimicrobials including antibiotics have been used for their control and treatment. However, probiotics or prebiotics or their combination offer a potential alternative intervention strategy for improving the host health and preventing foodborne pathogen colonization/infections in reservoir. Further, bioengineered probiotics expressing bioactive products to achieve specific function is highly desirable. Recently, we over-expressed mcra (myosin cross-reactive antigen) gene in Lactobacillus casei (Lc) and developed a bioengineered probiotics Lc + CLA which produce higher amounts of metabolites including conjugated linoleic acid (CLA). Furthermore, we also reported that prebiotic like components such as berry pomace (byproduct) phenolic extracts (BPEs) can enhance the growth of probiotics and improved the beneficial effects of probiotics. In this study, we evaluated the antimicrobial effect of modified Lc + CLA in combination of BPEs on growth, survival and pathogenesis of enterohemorrhagic Escherichia coli (EHEC).
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    BIOACTIVE LACTOBACILLUS CASEI IN REDUCING GROWTH AND COLONIZATION OF CAMPYLOBACTER JEJUNI
    (2018) Tabashsum, Zajeba; Biswas, Debabrata; Animal Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Campylobacter jejuni (CJ) is one of the pre-dominant causative agents of acute gastroenteritis in the US and occurs commonly through handling/consumption of contaminated poultry products. Probiotics with enhanced bioactive metabolites such as conjugated linoleic acids (CLAs) play crucial role in improving host health and act as antimicrobials. Further, prebiotic like components such as bioactive phenolics from berry pomace extract (BPE) can stimulate growth of beneficial microbes including Lactobacillus casei (LC) and inhibit bacterial pathogens in vitro. In this study, we aimed to assess efficiency of CLA overproducing LC (LC+mcra) alone or in presence of BPE against CJ. LC+mcra alone or LC+mcra with BPE reduced CJ growth, adhesion and invasion efficiency to cultured cells and also altered physicochemical properties, gene expressions related to virulence. These findings suggest, BPE and LC+mcra in combination may able to prevent CJ colonization in poultry and reduce cross-contamination, hence control foodborne infections with CJ in human.