Biology Theses and Dissertations

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    FIBER DIGESTION IN THE JUVENILE BLUE CRAB, CALLINECTES SAPIDUS RATHBUN
    (2006-01-24) Allman, Andrea Lauren; Place, Allen R; Marine-Estuarine-Environmental Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Five experiments were performed to determine the importance of chitin and cellulose in the diet of juvenile <em>C. sapidus</em>. A compartmentalized recirculating system was established to provide optimal conditions, maintaining the animals with little mortality. The appropriate ration, compartment size, and an adequate baseline diet were established. We replaced 20% of a commercial diet with varying amounts of chitin and cellulose. Crabs fed the cellulose-containing diet had higher growth rates, conversion efficiencies, molt increments and frequencies than crabs fed the chitin-containing diet, but were equal to the control diet. We then assayed for chitinase and cellulase in gut tissues. Chitinase had lower specific activity (0.072 + 0.159 mU mg-1min-1) than cellulase (3.52 + 0.16 mU mg-1min-1) in the foregut and hepatopancreas. There was no effect of diet on specific activity. The results show juvenile <em>C. sapidus</em> is capable of utilizing cellulose, but not chitin, when delivered as 20% of a diet.
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    DEGRADATION OF PLANT CELL WALL POLYSACCHARIDES BY SACCHAROPHAGUS DEGRADANS
    (2005-12-08) Taylor II, Larry Edmund; Weiner, Ronald M; Marine-Estuarine-Environmental Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    <em>Saccharophagus degradans</em> is an aerobic, Gram negative marine bacterium, isolated from decaying <em>Spartina alterniflora</em> in the Chesapeake Bay watershed. <em>S. degradans</em> can degrade and metabolize numerous complex polysaccharides, including the major components of the plant cell wall, cellulose, xylan and &#946;-glucan. Genomic analyses reveal that <em>S. degradans</em> has 77 genes coding for enzymes that are predicted to participate in the degradation of plant cell wall polysaccharides. These include complete, functional, multienzyme systems for the depolymerization of cellulose, xylan, arabinan, &#946;-1,3-glucans, &#946;-1,4-glucans, and mannan. Most of the cellulases are modular, some of which contain novel combinations of catalytic and/or substrate binding modules. In addition to its well-predicted plant wall degrading systems, <em>S. degradans</em> encodes 19 proteins which contain a carbohydrate binding module, but lack an identifiable catalytic domain and 12 glycanases for which function cannot be predicted by sequence analysis. Many of the plant wall degrading enzymes contain lipoprotein signature sequences, indicating that they are likely attached to the cell surface, thereby maintaining their reactions near the cell and preventing loss of enzyme or product to diffusion or competition. <em>S. degradans</em> is capable of using crystalline cellulose and intact plant matter as sole carbon and energy sources. Cellulose induces catalytic activity against all major plant cell wall polymers, suggesting a complex mechanism for coordinating the regulation of these multienzyme systems. In addition to its abundant carbohydrases, <em>S. degradans</em> encodes seven proteins with predicted molecular weights over 250,000 Daltons, one of which, CabA at 1,500,000 Daltons, is the largest known bacterial protein to date. These proteins contain calcium-binding repeat sequences suggesting a role in cell-tosurface adhesion or protein-to-protein interaction, perhaps as a means of surface enzyme attachment. These studies establish <em>S. degradans</em> as the first marine bacterium with a complete and functional cellulase system with the further ability to degrade plants in monoculture.