Plant Science & Landscape Architecture Theses and Dissertations
Permanent URI for this collectionhttp://hdl.handle.net/1903/2797
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Item Kinetics of Tetrachloroethene-Respiring Dehalobacter and Dehalococcoides Strains and Their Effects on Competition for Growth Substrates(2010) Lai, Yenjung; Becker, Jennifer G; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)The chlorinated solvents tetrachloroethene (PCE) and trichloroethene (TCE) are common groundwater contaminants. Reductive dechlorination of PCE and TCE at contaminated sites is commonly carried out by dehalorespiring bacteria that utilize these compounds as terminal electron acceptors, but often results in the accumulation of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC), rather than non-toxic ethene. This project focused on evaluating how interactions among dehalorespiring populations that may utilize the same electron acceptors, electron donors and/or carbon source may affect the extent of PCE dechlorination in situ. These interactions may be particularly important if both Dehalococcoides ethenogenes (Dhc. ethenogenes) and Dehalobacter restrictus (Dhb. restrictus) are present because these bacteria utilize the same electron donor (H2) and both respire PCE and TCE. However, unlike Dhc. ethenogenes, Dhb. restrictus cannot dechlorinate PCE beyond cDCE. Therefore, the outcome of the population interactions may determine the extent of detoxification achieved. Monod kinetic parameter estimates that describe chlorinated ethene and electron donor utilization by Dhc. ethenogenes and Dhb. restrictus at non-inhibitory substrate concentrations were obtained in batch assays. Substrate inhibition effects on both populations were also evaluated. Highly chlorinated ethenes negatively impacted dechlorination of the lesser chlorinated ethenes in both populations. In Dhc. ethenogenes, cometabolic transformation of VC was also inhibited by the presence of other chlorinated ethenes. PCE and TCE dechlorination by Dhb. restrictus was strongly inhibited by VC. The microbial interactions between Dhc. ethenogenes and Dhb. restrictus was investigated using reactors and mathematical models under engineered bioremediation and natural attenuation conditions. Under engineered bioremediation conditions, Dhc. ethenogenes became the dominant population, and the modeling predictions suggested that the inhibition of Dhb. restrictus by VC was a key factor in determining this outcome. Dechlorination rates by Dhb. restrictus appeared to be affected very little by low acetate concentrations under natural attenuation conditions, giving it an advantage over Dhc. ethenogenes, which requires relatively high acetate concentrations. This study highlighted that substrate interactions among dehalorespiring bacteria can influence their performance and contaminant fate under common bioremediation scenarios. A better understanding of the factors affecting the outcomes of these microbial interactions was achieved, which should aid in the design of successful bioremediation strategies.Item Utilization of Gypsum as a Filter Material in Agricultural Drainage Ditches: Impacts of Land Application on Soil Fertililty Conditions(2010) Grubb, Karen Lyn; McGrath, Joshua M.; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Agricultural drainage ditches can provide a direct connection between fields and surface waters, and some have been shown to deliver high loads of phosphorus (P) to sensitive water bodies. A potential way to reduce nutrient loads in drainage ditches is to install filter structures containing P sorbing materials (PSMs) including gypsum to remove P from ditch flow. One projected advantage would be the potential application of spent PSMs to agricultural fields to provide nutrients for crop production after the filter has lost its effectiveness. The study evaluated the feasibility of this strategy. Gypsum was saturated at two levels on mass basis of P, and applied to two soil types, a silt loam and a sandy loam and applied at both a high and low rate. The treated soils were incubated at 25° C, and samples were collected at 0, 1, 7, 28, 63, 91, 119, and 183 days after saturation.Item INVESTIGATING CRUMB RUBBER AMENDMENTS FOR EXTENSIVE GREEN ROOF SUBSTRATES(2010) Solano Torres, Sonia Lorelly; Lea-Cox, John D; Ristvey, Andrew G; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Extensive green roof systems can mitigate urban stormwater by capturing rainfall and reducing runoff volume. Green roof substrates, often made from expanded shales, slates and clays are fundamental for roof hydraulic dynamics, and for providing optimal plant growth conditions. However, these substrates occasionally impose load limitations for retrofitting existing infrastructure. This research studied recycled-tire crumb rubber, as a light-weight material for amending green roof substrates. Zinc release from crumb rubber was quantified, and the interactions with commercial rooflite® substrate and the effect of high Zn concentrations on the growth and uptake by Sedum were studied. Zn was found to leach from crumb rubber in quantities that could negatively affect plant growth; however, Zn was adsorbed onto cation exchange sites of the mineral and/or organic portion of rooflite®, preventing negative growth effects in Sedum. Crumb rubber could be utilized as an amendment with substrates having high cation exchange capacities.Item Redoximorphic Features Induced by Organic Amendments and Simulated Wetland Hydrology(2010) Gray, Adam Lincoln; Rabenhorst, Martin C; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)During wetland construction, it is common to add organic amendments to the soil, although little research has evaluated the effects of organic additions on the development of redoximorphic features. The objective of this study was to evaluate the effects of adding different types of organic materials, using different methods of incorporation, on the formation of redoximorphic features under hydric soil conditions. Five types of organic materials were incorporated into soil cores lacking redoximorphic features, using three incorporation methods. Cores were established as mesocosms in a controlled greenhouse environment or transplanted into a natural wetland. Mesocosms were periodically dissected and examined for newly formed redoximorphic features. The method of incorporating organic materials had a significant influence on the development of redoximorphic features, but the type of organic material had no significant effect. Organic materials should be concentrated into deeper zones during wetland construction to maximize development of redoximorphic features.Item HYDROMORPHOLOGY OF ANOMALOUS BRIGHT LOAMY SOILS ON THE MID-ATLANTIC COASTAL PLAIN(2009) Zurheide, Philip Klaus; Rabenhorst, Martin C; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Some loamy textured soils along the Mid-Atlantic coastal plain undergo extended periods of saturation or ponding, yet lack the hydromorphology that identifies them as hydric by any of the currently approved Field Indicators of Hydric Soils (FI). Termed Anomalous Bright Loamy Soils (ABLS), these were identified at four research sites on the Delmarva Peninsula. The hydrologic and biogeochemical status of these soils was monitored for three years along a hydrosequence at each site. A series of field and lab experiments were run to investigate the possible causes for the ABLS-phenomenon. The most likely cause is a combination of low hydrologic gradient coupled with the length of time since saturation. Using observed morphology, a newly developed Field Indicator successfully discriminated between five hydric soils that lacked an approved indicator and those that were not hydric. This indicator has now been approved as an official FI of Hydric Soils (F20).Item Pedogenesis, Inventory, and Utilization of Subaqueous Soils in Chincoteague Bay, Maryland(2007-11-28) Balduff, Danielle Marie; Rabenhorst, Martin C.; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Chincoteague Bay is the largest (19,000 ha) of Maryland's inland coastal bays bounded by Assateague Island to the east and the Maryland mainland to the west. It is connected to the Atlantic Ocean by the Ocean City inlet to the north and the Chincoteague inlet to the south. Water depth ranges mostly from 1.0 to 2.5 meters mean sea level (MSL). The objectives of this study were to identify the subaqueous landforms, evaluate the suitability of existing subaqueous soil-landscape models, develop a soils map, and demonstrate the usefulness of subaqueous soils information. Bathymetric data collected by the Maryland Geological Survey in 2003 were used to generate a digital elevation model (DEM) of Chincoteague Bay. The DEM was used, in conjunction with false color infrared photography to identify subaqueous landforms based on water depth, slope, landscape shape, depositional environment, and geographical setting (proximity to other landforms). The eight such landforms identified were barrier cove, lagoon bottom, mainland cove, paleo-flood tidal delta, shoal, storm-surge washover fan flat, storm-surge washover fan slope, and submerged headland. Previously established soil-landscape models were evaluated and utilized to create a soils map of the area. Soil profile descriptions were collected at 163 locations throughout Chincoteague Bay. Pedons representative of major landforms were characterized for a variety of chemical, physical and mineralogical properties. Initially classification using Soil Taxonomy (Soil Survey Staff, 2006) identified the major soils as Typic Sulfaquents, Haplic Sulfaquents, Sulfic Hydraquents, and Thapto-Histic Sulfaquents. Using a proposed modification to Soil Taxonomy designed to better accommodate subaqueous soils with the new suborder of Wassents, soils of Chincoteague Bay were primarily classified as Fluvic Sulfiwassents, Haplic Sulfiwassents, Thapto-Histic Sulfiwassents, Sulfic Hydrowassents, and Sulfic Psammowassents. To illustrate the application of subaqueous soils information, the suitability of soils for submerged aquatic vegetation (SAV) habitat was assessed, based upon past and current growth patterns in Chincoteague Bay and sediment properties known to affect SAV establishment and growth. The refined soil-landscape models and extensive soil characterization obtained in this study have advanced our understanding of subaqueous soils in coastal lagoon systems, and should prove valuable to coastal specialists managing these critical resources.Item Brassica Cover Crops for Nitrogen Retention in the Maryland Coastal Plain(2006-07-27) Dean, Jill Elise; Weil, Ray R; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)The Brassica cover crops, forage radish (Raphanus sativus L. cv 'Daichon'), oilseed radish (Raphanus sativus L. cv 'Adagio'), rape (Brassica napus L. cv 'Dwarf Essex'), and cereal rye (Secale cereale L. cv 'Wheeler') were examined for ability to decrease mineral N losses and influence organic N cycling at two Maryland Coastal Plain agricultural sites. Brassicas were similar or superior to rye regarding N uptake and soil profile NOsub3-N depletions (105-180 cm depth). Rape and rye maintained soil porewater NOsub3-N below 3 mg L to the minus 1 throughout spring while radish performed similarly on fine-textured soil, but caused porewater NOsub3-N > 10 mg L to the minus 1 on coarse-textured soil. Dissolved organic N averaged 51% of total N in porewater, but was unaffected by cover crops. Brassicas were as effective as rye in minimizing mineral N losses, but the role of cover crops in managing organic N was unclear.Item Utilizing Hybrid Poplar Trees to Phytoremediate Soils with Excess Phosphorus(2005-09-01) Neal, Amy; McIntosh, Marla S; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Phytoremediation, using plants to remove soil pollutants, has been suggested as a method to remove P from over-enriched soils. This research investigated the potential of utilizing hybrid poplar trees to remove excess P from soils associated with long-term poultry manure application. Hybrid poplar clones were planted in Snow Hill, MD, on three fields differing in previous poultry manure applications with Mehlich-3 soil-test P levels of 261, 478, and 982 mg P kg-1. During this two year study, soil P decreased on fields planted with hybrid poplar; the magnitude of the reduction was positively associated with initial soil-test P. Plant tissue P concentrations increased with soil P concentration. However, factors other than plant uptake were hypothesized to contribute to the soil-test P reductions. Results suggest that hybrid poplars have the potential to phytoremediate soils with excess P but that soil chemistry also impacts the fate of available P in the soil.Item WATER QUALITY IN MANAGEMENT INTENSIVE GRAZING AND CONFINED FEEDING DAIRY FARM WATERSHEDS(2005-07-12) Gilker, Rachel Esther; Weil, Ray R.; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Dairy farm size has increased in the United States, while the profit margin has decreased. An alternative to confined feeding dairy farming is management intensive grazing (MIG), a grass-based system relying on rotational grazing for most of the herd's dietary requirements. Previous research has measured high levels of nitrate leaching under MIG, citing the liquid nature and high nitrogen (N) content of urine. However, this research included heavy N fertilizer applications or was conducted on monolith lysimeters with artificial leaching processes and did not accurately represent mid-Atlantic MIG dairy farms. Phosphorus (P) losses have typically been attributed to runoff and erosion but are now being ascribed to leaching as well. To measure the magnitude of N and P losses to groundwater, we sampled shallow groundwater and pore water on one confined feeding and two MIG-based Maryland dairy farms between 2001 and 2004. Transects of nested piezometers and ceramic-tipped suction lysimeters were installed in two watersheds on each farm. Two streams running through two of the grazed watersheds were also sampled to measure the effects of grazing on surface water. For three years, groundwater and surface water samples were collected biweekly and pore water was collected when conditions made it possible. Samples were analyzed for inorganic N and dissolved reactive P and were digested for determination of dissolved organic N and P, pools previously not considered major sources of nutrient loss. Seasonal mean nitrate concentrations under the grazed watersheds remained below the EPA maximum contaminant load of 10 mg L-1 with only two exceptions on the grazed watersheds. Mean nitrate concentrations in the four grazed watersheds ranged from 3 to 7.44 mg L-1. Nitrogen losses were closely correlated to farm N surpluses. Groundwater P concentrations exceeded the EPA surface water critical levels in all six watersheds. Geologic factors, rather than dairy farm management, played a large role in P losses. In all watersheds, substantial pools of dissolved organic N and P were measured in groundwater. Low nitrate losses under MIG as well as the environmental advantages inherent in a grass-based system make grazing a viable Best Management Practice.Item Assessing evapotranspiration rates of a Mid-Atlantic red maple riparian wetland using sap flow sensors.(2005-04-13) Renz, Jennifer Theresa; Momen, Bahram; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Riparian forests are unique due to increased exposure of trees to winds and radiation and the subsequent effects on the quality and quantity of water discharge from the system. Since "edge effects" can enhance evapotranspiration (ET) of exposed trees, ET rates of a first-order red maple riparian wetland were assessed with thermal dissipation probes during the 2002 growing season to address: a) if edge trees transpire more water daily than interior trees, b) correlations among sap flow rates and energy balance-derived estimates, c) variations in ecosystem ET estimates based on 6 scaling variables, and d) diurnal correlations between maximum sap flow rates and streamflow losses. Results from this study indicate that: a) edge trees transpire more water daily than interior trees during early summer, b) choice of scaling variable affects estimation of ecosystem ET rates, and c) maximum sap flow rates correlate with streamflow losses diurnally under specific environmental conditions.