Environmental Science & Technology Theses and Dissertations
Permanent URI for this collectionhttp://hdl.handle.net/1903/2748
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Item Monitoring and Predicting the Microbial Water Quality in Irrigation Ponds(2022) Stocker, Matthew Daniel; Hill, Robert L; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Small- to medium-sized farm ponds are a popular source of irrigation water and provide a substantial volume of water for crop growth in the United States. The microbial quality of irrigation waters is assessed by measuring concentrations of the fecal indicator bacteria Escherichia coli (E. coli). Minimal guidance currently exists on the use of surface irrigation waters to minimize consumer health risks. The overall objective of this work was to provide science-based guidance for microbial water quality monitoring of irrigation ponds. Spatial and temporal patterns of E. coli were evaluated in two Maryland irrigation ponds over three years of observations. Patterns of E. coli were stable over the three years and found to be significantly correlated to patterns of water parameters such as temperature, dissolved oxygen, turbidity, and pH. The EPA Environmental Fluid Dynamics Code model was used to evaluate the spatial 3D heterogeneity of E. coli concentrations within the ponds. Significant differences in E. coli concentrations by sampling depth were found. Spatial heterogeneity of E. coli within the pond also resulted in substantial temporal variation at the irrigation pump, which was dependent on the intake location. Diurnal variation of E. coli concentrations was assessed for three farm ponds. E. coli concentrations declined from 9:00 to 15:00 for each pond, but statistically significant declines were only observed in two of the three ponds. Dissolved oxygen, pH, and electrical conductance were found to be the most influential environmental variables affecting E. coli concentrations. To better describe the relationships between E. coli and the environmental variables, four machine learning algorithms were used to estimate E. coli concentrations using water quality parameters as predictors. The random forest algorithm provided the highest predictive accuracy with R2 = 0.750 and R2 = 0.745 for Ponds 1 and 2, respectively, in the multi-year dataset containing 12 predictors. Temperature, electrical conductance, and organic matter content were identified as the most influential predictors. It is anticipated that the recommendations contained in this dissertation will be used to improve microbial monitoring strategies and protect public health.Item Release, Survival, And Removal of Bovine Manure-Borne Indicator Bacteria Under Simulated Rainfall(2017) Stocker, Matthew Daniel; Hill, Robert L; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)The effects of simulated rainfall intensities and its interactions with manure consistency and weathering on the release, survival, and removal of fecal indicator bacteria, Escherichia coli and enterococci, from land-applied dairy manure were evaluated. Rainfall intensity had significant effects on the number of bacteria in the soil following rainfall. Bacteria concentrations in soil decreased with increased soil depths and the topmost centimeter of soil accounted for the greatest proportion of bacteria. Escherichia coli persisted longer than enterococci once removed from manure. Manure consistency was not a significant factor in the removal of bacteria when manure was fresh, but as manure weathering progressed, consistency became a significant factor. The Vadas-Kleinman-Sharpley model was preferred over the exponential model for simulating the removal of manure-borne bacteria. Results of this work will be useful for improving predictions of the human health risks associated with manure-borne pathogenic microorganisms.Item Release and runoff/infiltration removal of Escherichia coli, enterococci, and total coliforms from land-applied dairy cattle manure(2014) Blaustein, Ryan Andrew; Hill, Robert L; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Simulating the rainfall-induced release of indicator bacteria from manure is essential to microbial fate and transport modeling with regard to water quality and food safety. Experiments were conducted to determine the effects of rainfall intensity, surface slope, and scale on the release of Escherichia coli, enterococci, and total coliforms from land-applied dairy manure. Rainfall intensity did not affect bacterial release dependencies on rainfall depth, but it did have a significant effect on the post-rainfall quantities of indicator bacteria in soil. While bacterial concentrations were evenly released into runoff and infiltration, the surface slope controlled the partitioning of total released bacterial loads. The proportion of E. coli released from manure exceeded enterococci, especially with infiltration flow. Scale had strong, inverse effects on the recovery of land-applied bacteria with runoff. These results will be used to improve microbial fate and transport models, critical for risk assessment of microbial contamination in the environment.