UMD Theses and Dissertations
Permanent URI for this collectionhttp://hdl.handle.net/1903/3
New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a given thesis/dissertation in DRUM.
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Item Patterns of oyster natural mortality in Chesapeake Bay, Maryland during 1991-2017 and its relationships with environmental factors and disease(2019) Doering, Kathryn Leah; Wilberg, Michael J; Marine-Estuarine-Environmental Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)A common method of estimating natural mortality in bivalves includes several assumptions that are likely violated for oysters Crassostrea virginica in Chesapeake Bay, Maryland. In addition, while oyster disease dynamics are well studied spatially and temporally in the mid-Atlantic region, changes in disease-related relationships have not been investigated in Maryland. We developed a Bayesian estimator for natural mortality and applied it to oysters in Maryland. We then used the model output along with environmental factors and disease data to explore changes in the disease system over time. We found the largest differences in natural mortality estimates between the box count method and Bayesian model 1-3 years after a high mortality event. Some relationships changed over time in the disease system, most notably those associated with MSX, suggesting resistance to MSX has potentially developed. This work improves our estimates of natural mortality and understanding of oyster disease dynamics in Maryland.Item The skin microbiome of woodland salamanders and its association with hosts' taxonomy, environment and health status(2016) Muletz Wolz, Carly Rae; Lips, Karen R.; Behavior, Ecology, Evolution and Systematics; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Microbial communities play central roles in animal health. Host species, environmental conditions and presence of pathogens can affect the diversity and composition of animal-associated microbiomes. Amphibians form integral and functionally important symbioses with microbes. The amphibian microbiome interacts with pathogens, and can protect hosts from disease, including the disease chytridiomycosis, caused by skin infection by Batrachochytrium dendrobatidis (Bd). The implications of amphibian-microbiome associations are difficult to predict because little is known about the factors shaping bacterial communities or their functional traits, such as anti-Bd properties. I used culture-dependent and culture-independent methods to characterize the skin microbiome of Plethodon salamanders in field and laboratory studies. I hypothesized that the evolutionary history, environmental conditions, and health status of the hosts shape skin bacterial community assemblages. In a field study, I sampled sympatric, congeneric salamander species (Plethodon cinereus, P. glutinosus, P. cylindraceus) across three localities to quantify the distribution of both anti-Bd bacteria and the entire bacterial community. I identified 50 anti-Bd bacterial OTUs and 480 bacterial OTUs overall on the salamander skin, with high prevalence and abundance of anti-Bd bacterial genera Pseudomonas, Acinetobacter and Stenotrophomonas. Within a locality, co-occurring salamanders generally had similar microbiome diversity and composition patterns, but these differed among sites. This indicates that environment is more influential in shaping skin microbiome patterns than differences in host properties in these species. I sampled P. cinereus along an elevational gradient, as a proxy for environmental variables that co-vary with elevation. Microbiome diversity and composition changed with elevation, in which compositional changes were related to soil pH. In a laboratory experiment, I quantified the responses of P. cinereus and the skin microbiome to temperature (13, 17, 21 °C) and pathogen (Bd+, Bd-) exposure to determine whether the native microbiome affected survival at natural temperatures. Temperature changed the microbiome, but this did not prevent host mortality from Bd. Instead, Bd exposure changed the microbiome and caused 78% mortality. My results demonstrate that environmental conditions and pathogen presence are important factors determining skin microbiome structure in Plethodon salamanders. These findings contribute to our understanding of animal-microbial symbioses, microbial community ecology, and amphibian disease ecology.Item The effects of co-varying diel-cycling hypoxia and pH on disease susceptibility, growth, and feeding in Crassostrea virginica(2014) Keppel, Andrew George; Breitburg, Denise L; North, Elizabeth W; Marine-Estuarine-Environmental Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Diel-cycling hypoxia and pH co-occur in shallow waters world-wide. Eutrophication tends to increase the occurrence and severity of diel cycles. We used laboratory experiments to investigate effects of diel-cycling DO and pH on acquisition and progression of infections by Perkinsus marinus, the protistan parasite which causes Dermo disease, as well as hemocyte activity, growth, and feeding in the eastern oyster, Crassostrea virginica, an important estuarine species. Diel-cycling DO increased P. marinus infection and cycling DO and pH stimulated hemocyte activity and reduced oyster growth. However, ambient environmental conditions and oyster age modulated some of these effects. Co-varying DO and pH cycles sometimes had less severe effects than either cycle independently. Oysters may acclimate to, or compensate for, effects of cycling conditions on growth. Variation in magnitude and spatial extent of cycling conditions is an important consideration when choosing restoration sites, as severe cycling conditions may hinder re-establishment of estuarine populations.Item THEATERS OF ANATOMY: DISEASED BODIES AND HISTORY WRITING IN THE HISPANIC TRANSATLANTIC WORLD(2011) Maus, Martha Ann; Merediz, Eyda M; Spanish Language and Literature; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)In this dissertation, entitled "Theaters of Anatomy: Diseased Bodies and History Writing in the Hispanic Transatlantic World," I establish a connection between medical and narrative disciplines using the famous medical treatise by Dr. Juan Huarte de San Juan, Examen de los ingenios para las ciencias, and three poetic/ history writing treatises, including Alonso López Pinciano's Philosophia Antigua Poética, Luis Cabrera de Córdoba's De Historia, para entenderla y escribirla, and Gerónimo de San José's Genio de la historia. I explore how the permeable boundaries between medical and narrative practices are woven together to create political meaning in a corpus of texts from the sixteenth century. By establishing the intricate relationship between medicine, political goals, and narrative; my work creates a historical trajectory between genres and show the way that each appropriates and differentiates itself from one another. It also demonstrates the parallel and dependent relationship on the developing studies of medicine and historiography. I use this interdisciplinary link to compare the figure of a history writer to that of a practitioner and follow the figure of the history-writer practitioner as he transforms through three texts: from a knight in Garci Rodríguez de Montalvo's Las Sergas de Esplandián, to a traveler in Álvar Núñez Cabeza de Vaca's Relación, and then as a friar in Bernardino de Sahagún's Historia general de las cosas de Nueva España. In each text, I investigate how physical disease is linked to sin, and thus non-Christians are portrayed as diseased. Throughout the dissertation, I explore how writers of history negotiate ways in which to heal the sinful and integrate them into a healthy Christian civic body.Item MOLECULAR CHARACTERIZATION OF INTERACTIONS BETWEEN TMV REPLICASE PROTEIN AND AUXIN RESPONSIVE PROTEINS: IMPLICATIONS IN DISEASE DEVELOPMENT(2006-11-25) Padmanabhan, Meenu Sreedevi; Culver, James; Cell Biology & Molecular Genetics; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Tobacco Mosaic Virus and Arabidopsis thaliana serve as ideal model systems to study the molecular aspects of virus - host interactions. Using this system, an interaction between the helicase domain within TMV replicase protein and an auxin responsive protein, IAA26 was identified. IAA26 is a member of the Aux/IAA family of transcription factors that function as repressors in signaling pathways controlled by the phytohormone auxin. Characterization of the interaction was carried out utilizing a helicase mutant defective in its interaction with IAA26 and by creating transgenic plants silenced for IAA26 expression. These studies suggest that the interaction was not essential for either viral replication or movement but promoted the development of disease symptoms. Cellular co-localization studies revealed that in TMV infected tissue, the nuclear localization and stability of IAA26 was compromised and the protein was relocalized to distinct cytoplasmic vesicles in association with the viral replicase. In keeping with its role as a transcription factor, the alterations in IAA26 function should lead to misregulation of downstream auxin responsive genes and this is supported by the fact that ~ 30% of the Arabidopsis genes displaying transcriptional alterations to TMV could be linked to the auxin signaling pathway. Aux/IAA family members share significant sequence and functional homology, and an additional interaction screen identified two more Arabidopsis Aux/IAA proteins, IAA27 and IAA18 and a putative tomato Aux/IAA protein, LeIAA26 that could interact with TMV helicase. The nuclear localization of these three proteins was disrupted by TMV and alterations in LeIAA26 levels induced virus infection-like symptoms in tomato. Additionally, transgenic plants over-expressing a proteolysis resistant mutant of IAA26 showed abnormal developmental phenotype, the severity of which was abrogated during TMV infection which blocked nuclear accumulation of the protein. Taken together, these findings suggest that TMV induced disease symptoms can partially be explained by the ability of the virus to disrupt the functioning of interacting Aux/IAA proteins within susceptible hosts. The significance of such interactions is yet to be determined but it appears that they may be advantageous to the virus while infecting tissues that are in a developmentally static stage.