UMD Theses and Dissertations
Permanent URI for this collectionhttp://hdl.handle.net/1903/3
New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a given thesis/dissertation in DRUM.
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Item Effects of post-harvest management practices on the degradation of Bacillus thuringiensis proteins in genetically modified corn residue(2018) Johnson, Veronica; Hooks, Cerruti RR; Entomology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Most studies addressing the ecological effects of Bt crops have focused on non-target effects during the crop growing cycle. Less information is available on the fate of expressed toxins in crop residue after harvest in a no-till production system. This research investigated the effects of four post-harvest management practices on the degradation rates of Cry proteins expressed in SmartStax field corn. Cry protein degradation expressed as growth inhibition of Ostrinia nubilalis larvae after harvest was measured by a feeding bioassay, and enzyme-linked immunosorbent assays were used to detect the presence of Cry proteins. Cry proteins retained significant levels of biological activity at crop senescence and were still present in corn residue for more than 20 weeks after harvest. Despite inconsistencies in treatment effects, the study demonstrated that post-harvest practices that increase soil-residue contact increase protein degradation, thereby reducing the period of exposure for non-target organisms.Item Bt GENETICS EFFECT ON CORN HYBRID PERFORMANCE: A COMPARISON OF TWO NEAR ISOLINE CORN HYBRIDS(2018) Thorne, Louis; Kratochvil, Robert J; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Most corn (Zea mays L.) hybrids planted in the U.S. are the result of genetic modification that gives them a Bt gene or genes obtained from the bacterium, Bacillus thuringiensis (Berliner) (Bt), that express insecticidal proteins and enables these hybrids to be resistant to several insects. European corn borer (ECB) (Ostrinia nubilalis, Hübner) is the main Lepidopteran pest targeted by the Bt corn technology. All Bt events used in current corn hybrids provide 100% control of ECB. This has led to widespread use of Bt hybrids and has resulted in a drastic decrease in the ECB population. This raises the question whether it is still economically feasible to plant Bt hybrids that have higher seed costs in environments where the ECB pest level is low. The objectives of this study were: 1) compare the yield and agronomic performance of a pair of corn near-isoline hybrids with and without the ¬Bt traits; and 2) evaluate the agronomic and economic optimums for yield and nitrogen (N) rate for each near-isoline hybrids. A two-year study at three University of Maryland research farms in 2013-2014 examined each hybrid type for stalk damage due to ECB, yield performance, the optimum N rate for maximizing yield, and the economic returns the two hybrids provided. This study found minimal ECB stalk damage and no consistent agronomic or economic yield difference between the Bt and non-Bt hybrids. Neither hybrid type was determined to have a consistent nitrogen use efficiency (NUE) advantage. The results of this study indicate that producers should not have concerns over hybrid type choice, now that there is significant regional suppression of ECB below economic levels.Item AN ECOLOGICAL RISK ASSESSMENT OF BT TRANSGENIC SWEET CORN ON NON-TARGET ARTHROPOD COMMUNITIES(2005-04-28) Rose, Robyn I.; Dively, Galen P; Entomology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)An ecological risk assessment evaluating potential adverse affects to non-target arthropods is necessary when releasing a novel transgenic crop. Field studies were conducted to assess tritrophic level effects of insecticide treated and untreated Bt and non-Bt sweet corn hybrids on the abundances, species diversity and functional processes of foliage-dwelling, aerial and soil surface arthropods. The diversity and abundance of decomposers, predators and parasitoids were determined by plant inspections, sticky card, and pitfall trap sampling for two years at two Maryland locations. Functional processes including predation, parasitism, reproduction and colonization were evaluated at one location over three years. Predation rates were estimated from sentinel egg masses of a lepidopteran; levels of parasitism were measured from naturally-occurring chrysopid eggs; emergence traps determined the recruitment rates of arthropods emerging from soil-litter; and litterbags measured arthropod colonization and litter degradation rates. Beneficial arthropod communities and functional processes were not significantly affected by exposure to Bt sweet corn. Insecticides significantly reduced arthropod diversity and abundance as well as the rate of predation, parasitism, colonization and recruitment of organisms. Less disruption occurred in Bt plots treated once than compared with non-Bt plots treated five times. Since the number of insecticide applications are reduced, planting Bt sweet corn will result in benefits to non-target arthropods and ecological processes. A second aspect of the ecological risk assessment focused on laboratory and field studies to evaluate the effects of Bt pollen on honey bee survival, brood development and foraging behavior. Results also were used to examine the statistical power of study protocols. Laboratory tests showed no effects of feeding on Bt pollen on survival or hypopharyngeal gland development measured indirectly by head weight gain of newly-emerged bees. In field studies conducted for two years at three locations, colonies foraging in sweet corn plots and fed Bt pollen cakes for 28 days showed no adverse effects on bee weight, hive strength, brood development and foraging behavior. Foraging bee weight, number of foragers returning with pollen loads, pollen load weight, and brood size were the most reliable endpoints to examine for non-target effects on the general fitness of colonies.