UMD Theses and Dissertations

Permanent URI for this collectionhttp://hdl.handle.net/1903/3

New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a given thesis/dissertation in DRUM.

More information is available at Theses and Dissertations at University of Maryland Libraries.

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Author: search.filters.author.Schoenberg, Katie MarieSubject: search.filters.subject.mouse

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    Nutritional and Physiological Control of Metabolic Pathways That Alter Milk Protein and Lactose Synthesis by the Mammary Gland
    (2006-12-08) Schoenberg, Katie Marie; Bequette, Brian J; Animal Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    The objectives of this research were to develop a [U-<sup>13</sup>C]glucose tracer approach establishing the pathways and substrates for milk lactose and casein synthesis, and determine the influence of protein intake on murine mammary gland metabolism. Milk samples were collected after one, three and five days of feeding tracer (as 10% of dextrose). <sup>13</sup>C-Isotopic and isotopomer plateaus were attained by day three, establishing the time-course necessary for tracer feeding. 23% of lactose-derived glucose originated from sources other than blood glucose. Six paired (intake and pups equal) sets of lactating mice were fed either a normal (20%) or low (10%) protein diet. <sup>13</sup>C-mass isotopomer distribution (MID) in lactose-derived glucose and galactose did not differ, indicating common mammary metabolic pools. <sup>13</sup>C-MID in blood versus casein indicated significant mammary synthesis of glutamate (Normal:51%, Low:50%), alanine (Normal:32%, Low:29%), and serine (Normal:18%, Low:37%, P < 0.05), suggesting additional requirements for glucose and/or EAA for NEAA synthesis.