UMD Theses and Dissertations

Permanent URI for this collectionhttp://hdl.handle.net/1903/3

New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a given thesis/dissertation in DRUM.

More information is available at Theses and Dissertations at University of Maryland Libraries.

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Author: search.filters.author.Kanani, Harin HaridasSubject: search.filters.subject.Biology, Plant Physiology

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    TIME SERIES METABOLIC PROFILING ANALYSIS OF THE SHORT TERM Arabidopsis thaliana RESPONSE TO ELEVATED CO2 USING GAS CHROMATOGRAPHY MASS SPECTROMETRY.
    (2004-08-30) Kanani, Harin Haridas; Klapa, Maria I; Chemical Engineering; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Metabolic profiling has emerged as a high throughput technique for the quantitative analysis of the cellular physiological state at the metabolic level. It allows for the simultaneous relative quantification of hundreds of low molecular weight intra cellular metabolites. In this analysis, the polar metabolic profiles of A. thaliana liquid cultures (grown for 12 days, under light and 23°C) throughout 1-day treatment with 1% CO2, were measured using gas chromatography-mass spectrometry. Despite the advantages of time series analysis, this is the first plant metabolic profiling study of this type reported in the literature. The time series metabolic profiles were analyzed using multivariate statistical techniques. Data analysis revealed repression of photorespiration, repression of nitrogen assimilation and increase in structural carbohydrates. It is for the first time that the latter phenomenon is observed as a result of elevated CO2 in the plant environment.