Gemstone Team Research

Permanent URI for this collectionhttp://hdl.handle.net/1903/9070

The Gemstone Program at the University of Maryland is a unique multidisciplinary four-year research program for selected undergraduate honors students of all majors. Under guidance of faculty mentors and Gemstone staff, teams of students design, direct and conduct significant research, often but not exclusively exploring the interdependence of science and technology with society. Gemstone students are members of a living-learning community comprised of fellow students, faculty and staff who work together to enrich the undergraduate experience. This community challenges and supports the students in the development of their research, teamwork, communication and leadership skills. In the fourth year, each team of students presents its research in the form of a thesis to experts, and the students complete the program with a citation and a tangible sense of accomplishment.

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Author: search.filters.author.Axe, JenniferSubject: search.filters.subject.alternative energy

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    Harvesting life's energy: increase in the aerotolerence of the electrogenic anaerobe geobacter sulfurreducens due to over-expression of superoxide dismutase and catalase
    (2009-05) Axe, Jennifer; Billmyre, R. Blake; Duty, Kevin Heffner; Hitz, Greg; Trager, Lauren; Weatherford, Allison
    Geobacter-based microbial fuel cells are becoming increasingly viable as a source of alternative energy. Current research and commercial application have been slowed by the inability of Geobacter species to tolerate the presence of oxygen. Oxidative stress protection enzymes normally play a key role in protecting cells from oxygen damage. This project hypothesizes that the over-expression of two important oxidative stress protection enzymes, catalase and superoxide dismutase, can increase aerotolerance. These genes were amplified from the genome of Geobacter sulfurreducens and cloned into plasmid pRG5 behind the ptaclac promoter. This plasmid was transformed into both E. coli and G. sulfurreducens to examine oxygen tolerance, gene expression, and enzyme activity. Preliminary data show increases in enzyme activity in E. coli and an increase in aerotolerance in G. sulfurreducens for both genes. This seems to be the first example of an increase in an obligate anaerobe's aerotolerance due to the intentional introduction of oxidative stress genes.