Next-generation Mass Spectrometry With Multi-omics For Discoveries In Cell And Neurodevelopmental Biology

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dc.contributor.advisorNemes, Peteren_US
dc.contributor.authorLi, Jieen_US
dc.contributor.departmentChemistryen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2023-10-06T05:34:32Z
dc.date.available2023-10-06T05:34:32Z
dc.date.issued2022en_US
dc.description.abstractUnderstanding tissue formation advances our understanding of the causes of disease and the obtained knowledge can be potentially applied to develop personalized interventions. However, to explore the underlying mechanisms that govern tissue formation, there is a high and unmet need to develop new technologies to characterize different types of biomolecules from early-stage embryonic precursor cells and their descendent cells during development. This dissertation discusses new technological advancements to facilitate multi-omic (proteomic and metabolomic) analysis to explore cell-to-cell differences and uncover mechanisms underlying tissue formation. The work presented herein illustrates the development of in vivo microsampling and single-cell mass spectrometry (MS) to uncover cell heterogeneity among embryonic cells. Additionally, this dissertation work studies the biological role of metabolites in cell fate determination by exploring the mechanisms underlying metabolite-induced cell fate change. Moreover, this work introduces a novel technique called MagCar developed to track and isolate tissue-specific cells at later stages, which enables studying temporal molecular changes to gain new information about tissue formation.en_US
dc.identifierhttps://doi.org/10.13016/dspace/lhne-ywsl
dc.identifier.urihttp://hdl.handle.net/1903/30731
dc.language.isoenen_US
dc.subject.pqcontrolledChemistryen_US
dc.subject.pqcontrolledAnalytical chemistryen_US
dc.subject.pqcontrolledDevelopmental biologyen_US
dc.subject.pquncontrolledCell and developmental biologyen_US
dc.subject.pquncontrolledMass spectrometryen_US
dc.subject.pquncontrolledMulti-omicsen_US
dc.subject.pquncontrolledSamplingen_US
dc.subject.pquncontrolledSeparationen_US
dc.subject.pquncontrolledTissue formationen_US
dc.titleNext-generation Mass Spectrometry With Multi-omics For Discoveries In Cell And Neurodevelopmental Biologyen_US
dc.typeDissertationen_US

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