Novel Modular Rhodopsins from Green Algae Hold Great Potential for Cellular Optogenetic Modulation Across the Biological Model Systems

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dc.contributor.authorAwasthi, Mayanka
dc.contributor.authorSushmita, Kumari
dc.contributor.authorKaushik, Manish Singh
dc.contributor.authorRanjan, Peeyush
dc.contributor.authorKateriya, Suneel
dc.date.accessioned2023-11-08T16:48:48Z
dc.date.available2023-11-08T16:48:48Z
dc.date.issued2020-10-28
dc.description.abstractLight-gated ion channel and ion pump rhodopsins are widely used as optogenetic tools and these can control the electrically excitable cells as (1) they are a single-component system i.e., their light sensing and ion-conducting functions are encoded by the 7-transmembrane domains and, (2) they show fast kinetics with small dark-thermal recovery time. In cellular signaling, a signal receptor, modulator, and the effector components are involved in attaining synchronous regulation of signaling. Optical modulation of the multicomponent network requires either receptor to effector encoded in a single ORF or direct modulation of the effector domain through bypassing all upstream players. Recently discovered modular rhodopsins like rhodopsin guanylate cyclase (RhoGC) and rhodopsin phosphodiesterase (RhoPDE) paves the way to establish a proof of concept for utilization of complex rhodopsin (modular rhodopsin) for optogenetic applications. Light sensor coupled modular system could be expressed in any cell type and hence holds great potential in the advancement of optogenetics 2.0 which would enable manipulating the entire relevant cell signaling system. Here, we had identified 50 novel modular rhodopsins with variant domains and their diverse cognate signaling cascades encoded in a single ORF, which are associated with specialized functions in the cells. These novel modular algal rhodopsins have been characterized based on their sequence and structural homology with previously reported rhodopsins. The presented novel modular rhodopsins with various effector domains leverage the potential to expand the optogenetic tool kit to regulate various cellular signaling pathways across the diverse biological model systems.
dc.description.urihttps://doi.org/10.3390/life10110259
dc.identifierhttps://doi.org/10.13016/dspace/jf0e-pkec
dc.identifier.citationAwasthi, M.; Sushmita, K.; Kaushik, M.S.; Ranjan, P.; Kateriya, S. Novel Modular Rhodopsins from Green Algae Hold Great Potential for Cellular Optogenetic Modulation Across the Biological Model Systems. Life 2020, 10, 259.
dc.identifier.urihttp://hdl.handle.net/1903/31306
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCell Biology & Molecular Geneticsen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectenzyme-rhodopsin
dc.subjectchannelrhodopsins
dc.subjectoptogenetics
dc.subjecttwo-component system
dc.subjectcyclase
dc.subjectphosphodiesterase
dc.titleNovel Modular Rhodopsins from Green Algae Hold Great Potential for Cellular Optogenetic Modulation Across the Biological Model Systems
dc.typeArticle
local.equitableAccessSubmissionNo

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