IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts

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dc.contributor.authorHoover, Lisa I.
dc.contributor.authorFredericksen, Brenda L.
dc.date.accessioned2024-01-23T19:16:31Z
dc.date.available2024-01-23T19:16:31Z
dc.date.issued2014-03-24
dc.description.abstractAlthough dermal fibroblasts are one of the first cell types exposed to West Nile virus (WNV) during a blood meal by an infected mosquito, little is known about WNV replication within this cell type. Here, we demonstrate that neuroinvasive, WNV-New York (WNV-NY), and nonneuroinvasive, WNV-Australia (WNV-AUS60) strains are able to infect and replicate in primary human dermal fibroblasts (HDFs). However, WNV-AUS60 replication and spread within HDFs was reduced compared to that of WNV-NY due to an interferon (IFN)-independent reduction in viral infectivity early in infection. Additionally, replication of both strains was constrained late in infection by an IFN-β-dependent reduction in particle infectivity. Overall, our data indicates that human dermal fibroblasts are capable of supporting WNV replication; however, the low infectivity of particles produced from HDFs late in infection suggests that this cell type likely plays a limited role as a viral reservoir in vivo.
dc.description.urihttps://doi.org/10.3390/v6031424
dc.identifierhttps://doi.org/10.13016/dspace/dz2y-lqj9
dc.identifier.citationHoover, L.I.; Fredericksen, B.L. IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts. Viruses 2014, 6, 1424-1441.
dc.identifier.urihttp://hdl.handle.net/1903/31598
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCell Biology & Molecular Geneticsen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtCollege of Computer, Mathematical & Natural Sciencesen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectWest Nile Virus
dc.subjectinterferon
dc.subjecthuman dermal fibroblast
dc.subjectantiviral response
dc.subjectintracellular innate immunity
dc.titleIFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts
dc.typeArticle
local.equitableAccessSubmissionNo

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