Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote

Simple item page
dc.contributor.authorEisen, Jonathan A.
dc.contributor.authorCoyne, Robert S.
dc.contributor.authorWu, Martin
dc.contributor.authorWu, Dongying
dc.contributor.authorThiagarajan, Mathangi
dc.contributor.authorWortman, Jennifer R.
dc.contributor.authorBadger, Jonathan H.
dc.contributor.authorRen, Qinghu
dc.contributor.authorAmedeo, Paolo
dc.contributor.authorJones, Kristie M.
dc.contributor.authorTallon, Luke J.
dc.contributor.authorDelcher, Arthur L.
dc.contributor.authorSalzberg, Steven L.
dc.contributor.authorSilva, Joana C.
dc.contributor.authorHaas, Brian J.
dc.contributor.authorMajoros, William H.
dc.contributor.authorFarzad, Maryam
dc.contributor.authorCarlton, Jane M.
dc.contributor.authorSmith, Robert K. Jr.
dc.contributor.authorGarg, Jyoti
dc.contributor.authorPearlman, Ronald E.
dc.contributor.authorKarrer, Kathleen M.
dc.contributor.authorSun, Lei
dc.contributor.authorManning, Gerard
dc.contributor.authorElde, Nels C.
dc.contributor.authorTurkewitz, Aaron P.
dc.contributor.authorAsai, David J.
dc.contributor.authorWilkes, David E.
dc.contributor.authorWang, Yufeng
dc.contributor.authorCai, Hong
dc.contributor.authorCollins, Kathleen
dc.contributor.authorStewart, B. Andrew
dc.contributor.authorLee, Suzanne R.
dc.contributor.authorWilamowsk, Katarzyna
dc.contributor.authorWeinberg, Zasha
dc.contributor.authorRuzzo, Walter L.
dc.contributor.authorWloga, Dorota
dc.contributor.authorGaertig, Jacek
dc.contributor.authorFrankel, Joseph
dc.contributor.authorTsao, Che-Chia
dc.contributor.authorGorovsky, Martin A.
dc.contributor.authorKeeling, Patrick J.
dc.contributor.authorWaller, Ross F.
dc.contributor.authorPatron, Nicola J.
dc.contributor.authorCherry, J. Michael
dc.contributor.authorStover, Nicholas A.
dc.contributor.authorKrieger, Cynthia J.
dc.contributor.authordel Toro, Christina
dc.contributor.authorRyder, Hilary F.
dc.contributor.authorWilliamson, Sondra C.
dc.contributor.authorBarbeau, Rebecca A.
dc.contributor.authorHamilton, Eileen P.
dc.contributor.authorOrias, Eduardo
dc.date.accessioned2008-06-05T19:16:21Z
dc.date.available2008-06-05T19:16:21Z
dc.date.issued2006
dc.description.abstractThe ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. The germline-like micronucleus (MIC) has its genome held in reserve for sexual reproduction. The soma-like macronucleus (MAC), which possesses a genome processed from that of the MIC, is the center of gene expression and does not directly contribute DNA to sexual progeny. We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. thermophila, which is approximately 104 Mb in length and composed of approximately 225 chromosomes. Overall, the gene set is robust, with more than 27,000 predicted protein-coding genes, 15,000 of which have strong matches to genes in other organisms. The functional diversity encoded by these genes is substantial and reflects the complexity of processes required for a free-living, predatory, single-celled organism. This is highlighted by the abundance of lineage-specific duplications of genes with predicted roles in sensing and responding to environmental conditions (e.g., kinases), using diverse resources (e.g., proteases and transporters), and generating structural complexity (e.g., kinesins and dyneins). In contrast to the other lineages of alveolates (apicomplexans and dinoflagellates), no compelling evidence could be found for plastid-derived genes in the genome. UGA, the only T. thermophila stop codon, is used in some genes to encode selenocysteine, thus making this organism the first known with the potential to translate all 64 codons in nuclear genes into amino acids. We present genomic evidence supporting the hypothesis that the excision of DNA from the MIC to generate the MAC specifically targets foreign DNA as a form of genome self-defense. The combination of the genome sequence, the functional diversity encoded therein, and the presence of some pathways missing from other model organisms makes T. thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance.en
dc.format.extent1882784 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationMacronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote. J.A. Eisen, et al. PLoS Biology 4:9 (2006): e286.en
dc.identifier.urihttp://hdl.handle.net/1903/7987
dc.language.isoen_USen
dc.publisherPLoS Biologyen
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtComputer Scienceen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectTetrahymena thermophilaen
dc.subjectciliateen
dc.subjectmolecular biologyen
dc.subjectcellular biologyen
dc.subjectgermline-like micronucleus (MIC)en
dc.subjectsoma-like macronucleus (MAC)en
dc.subjectsexual progenyen
dc.subjectgenesen
dc.subjectDNAen
dc.subjectlineage-specific duplicationsen
dc.titleMacronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryoteen
dc.typeArticleen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PLoS.pdf
Size:
1.8 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.8 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Computer Science Research Works