The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle

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dc.contributor.authorCong, Xiaofei
dc.contributor.authorDoering, Jonathan
dc.contributor.authorMazala, Davi A. G.
dc.contributor.authorChin, Eva R.
dc.contributor.authorGrange, Robert W.
dc.contributor.authorJiang, Honglin
dc.date.accessioned2021-07-28T16:24:11Z
dc.date.available2021-07-28T16:24:11Z
dc.date.issued2016-04-11
dc.description.abstractThe SH3 and cysteine-rich domain 3 (Stac3) gene is specifically expressed in the skeletal muscle. Stac3 knockout mice die perinatally. In this study, we determined the potential role of Stac3 in postnatal skeletal muscle growth, fiber composition, and contraction by generating conditional Stac3 knockout mice. We disrupted the Stac3 gene in 4-week-old male mice using the Flp-FRT and tamoxifen-inducible Cre-loxP systems. RT-qPCR and western blotting analyses of the limb muscles of target mice indicated that nearly all Stac3 mRNA and more than 70 % of STAC3 protein were deleted 4 weeks after tamoxifen injection. Postnatal Stac3 deletion inhibited body and limb muscle mass gains. Histological staining and gene expression analyses revealed that postnatal Stac3 deletion decreased the size of myofibers and increased the percentage of myofibers containing centralized nuclei, with no effect on the total myofiber number. Grip strength and grip time tests indicated that postnatal Stac3 deletion decreased limb muscle strength in mice. Muscle contractile tests revealed that postnatal Stac3 deletion reduced electrostimulation-induced but not the ryanodine receptor agonist caffeine-induced maximal force output in the limb muscles. Calcium imaging analysis of single flexor digitorum brevis myofibers indicated that postnatal Stac3 deletion reduced electrostimulation- but not caffeine-induced calcium release from the sarcoplasmic reticulum. This study demonstrates that STAC3 is important to myofiber hypertrophy, myofiber-type composition, contraction, and excitation-induced calcium release from the sarcoplasmic reticulum in the postnatal skeletal muscle.en_US
dc.description.urihttps://doi.org/10.1186/s13395-016-0088-4
dc.identifierhttps://doi.org/10.13016/ebat-iu6c
dc.identifier.citationCong, X., Doering, J., Mazala, D.A.G. et al. The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle. Skeletal Muscle 6, 17 (2016).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27587
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtSchool of Public Healthen_us
dc.relation.isAvailableAtKinesiologyen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectSkeletal muscleen_US
dc.subjectHypertrophyen_US
dc.subjectFiber typeen_US
dc.subjectCalcium releaseen_US
dc.titleThe SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscleen_US
dc.typeArticleen_US

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