Mutant Transformation of Pathogenic Neisseria Strains

dc.contributor.authorAjudua, Emmanuel
dc.date.accessioned2012-04-19T15:53:36Z
dc.date.available2012-04-19T15:53:36Z
dc.date.issued2011
dc.description.abstractNeisseria gonorrhoeae is a bacterium that produces ~700,000 new gonococcal diseases in the US each year. While gonorrhea is localized to the urethra in men, in women it can spread to the cervix, uterus and fallopian tubes. Lipooligosaccharide (LOS) is a surface antigen embedded in the outer membrane of all Neisseria strains that causes disease. However, with commensal Neisseria strains disease does not occur. The commensal strains, with the exception of two, all contain a third heptose attached to their core. We hypothesized that if a third heptose could be added to the LOS Beta chains of Neisseria gonorrhoeae, then the LOS would become less virulent. The proposed gene sequence that codes for the HepIII transferase (enzyme that adds heptose) was located. The DNA was extracted and run in a PCR reaction to amplify the proposed gene sequence, which was then purified. PLES2 plasmid DNA was introduced to competent DH5aMCR cells by heat shock transformation to make multiple copies. The resultant cells were plated onto LB+Ampicillin+Xgal for selectivity and restreaked for growth. As research continues the plasmid will be isolated from the E. coli and the purified gene will be inserted into the plasmid. After a few tests, the plasmid with the gene will be inserted into Neisseria gonorrhoeae to observe effects.en_US
dc.identifier.citationThe University of Maryland McNair Scholars Undergraduate Research Journal, Vol. 3, 2011: 25-33.en_US
dc.identifier.urihttp://hdl.handle.net/1903/12453
dc.language.isoen_USen_US
dc.relation.isAvailableAtRonald E. McNair Post-Baccalaureate Degree Program
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtUniversity of Maryland (College Park, Md.)
dc.subjectgonorrheaen_US
dc.subjectgeneticsen_US
dc.subjecttransformationen_US
dc.titleMutant Transformation of Pathogenic Neisseria Strainsen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Ajudua.pdf
Size:
360.68 KB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.57 KB
Format:
Item-specific license agreed upon to submission
Description: