A prevention program that promotes attitudinal and behavioral change has been repeatedly recommended as an effective measure for community prevention and control of HIV/AIDS. Within the past decade, disease control programs among African Americans have shown no significant changes, as HIV/AIDS and related infections continue as the leading causes of death and disability in this population. It is particularly acute among African American females who, although representing only 13.9% of the United States female population, presently account for over 60% of all AIDS cases among females in the United States. The epidemic nature of the disease in this group poses the issue of African American women’s rights and underscores their physical, emotional and sociocultural vulnerability to HIV/AIDS. This study used an open-ended attitude, knowledge, feelings, and behavior questionnaire (AKFBQ) survey approach to assess knowledge and various HIV/AIDS high-risk attitudinal and behavioral factors that affect the incidence, mortality, and morbidity of HIV/AIDS amongwomen in specific urban centers and rural communities in the United States. Data analysis showed no differences in factors favoring rate of infection and level of spread of HIV/AIDS among the women in both the urban and rural communities. Although level of knowledge was higher among the urban subjects than rural ones, their beliefs, attitude/feelings, and potential for behavioral change did not differ significantly among women in the two communities. This article advocates both an ethnically sensitive and gender-specific HIV/AIDS intervention program for African American women and a more active involvement in and participation of African American leaders, parents, and faith-based communities in AIDS control efforts for African Americans.