School of Public Health

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The collections in this community comprise faculty research works, as well as graduate theses and dissertations.

Note: Prior to July 1, 2007, the School of Public Health was named the College of Health & Human Performance.

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Subject: search.filters.subject.antibiotic resistance

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Now showing 1 - 4 of 4
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    Occupational Exposure to Staphylococcus aureus and Enterococcus spp. among Spray Irrigation Workers Using Reclaimed Water
    (MDPI, 2014-04-17) Rosenberg Goldstein, Rachel E.; Micallef, Shirley A.; Gibbs, Shawn G.; He, Xin; George, Ashish; Sapkota, Amir; Joseph, Sam W.; Sapkota, Amy R.
    As reclaimed water use expands, it is important to evaluate potential occupational health risks from exposure to this alternative water source. We compared odds of colonization with methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus (MSSA), vancomycin-resistant enterococci (VRE), and vancomycin-susceptible enterococci (VSE) between spray irrigation workers using reclaimed water and office worker controls. Nasal and dermal swabs from 19 spray irrigation workers and 24 office worker controls were collected and analyzed for MRSA, MSSA, VRE, and VSE. Isolates were confirmed using standard biochemical tests and polymerase chain reaction assays. Antimicrobial susceptibility testing was performed by Sensititre® microbroth dilution. Data were analyzed by two-sample proportion, chi-square, Fisher’s exact tests, and logistic regression. No MRSA or VRE were detected in any samples. MSSA was detected in 26% and 29% of spray irrigators and controls, respectively. VSE was detected in 11% and 0% of spray irrigation workers and controls, respectively. The adjusted odds of MSSA, multidrug-resistant MSSA, and either MSSA or VSE colonization were greater among spray irrigation workers, however results were not statistically significant. Future studies with larger sample sizes are needed to further evaluate this relationship.
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    Assessing the Presence of Antibiotic-Resistant Enterococcus in Reclaimed Water Used for Spray Irrigation
    (2013) Carey, Stephanie Ann; Sapkota, Amy R; Turner, Paul; Maryland Institute for Applied Environmental Health; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Reclaiming wastewater is increasing in the US to combat dwindling freshwater supplies. This water potentially contains pathogenic bacteria; therefore, we evaluated the occurrence, concentration, and antimicrobial susceptibilities of Enterococcus spp.--an important opportunistic pathogen that remains a leading cause of nosocomial infections--in reclaimed water used for spray irrigation (SI). A total of 48 wastewater effluent and SI samples were collected in 2009 and 2010 from the Mid-Atlantic and Midwest regions of the US. Enterococci were isolated using membrane filtration, confirmed using biochemical tests and PCR, and tested for antimicrobial susceptibility using the Sensititre® dilution system. We detected total enterococci and vancomycin-resistant enterococci (VRE) in 68% (27/40) and 8% (3/40), respectively, of all SI samples. VRE and vancomycin-intermediate enterococci (VIE) represented 2% (1/41) and 10% (4/41), respectively, of the total enterococci recovered from all SI sites. Our findings show that SI workers may be exposed to enterococci during spray irrigation activities.
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    Self-medication with antibiotics for the treatment of menstrual symptoms in southwest Nigeria: a cross-sectional study
    (2010-10-15) Sapkota, Amy R; Coker, Morenike E; Rosenberg Goldstein, Rachel E; Atkinson, Nancy L; Sweet, Shauna J; Sopeju, Priscilla O; Ojo, Modupe T; Otivhia, Elizabeth; Ayepola, Olayemi O; Olajuyigbe, Olufunmiso O; Shireman, Laura; Pottinger, Paul S; Ojo, Kayode K
    Background: Self-medication with antibiotics is an important factor contributing to the development of bacterial antibiotic resistance. The purpose of this study was to evaluate the prevalence of self-medication with antibiotics for the treatment of menstrual symptoms among university women in Southwest Nigeria. Methods: A cross-sectional survey was administered to female undergraduate and graduate students (n = 706) at four universities in Southwest Nigeria in 2008. The universities were selected by convenience and the study samples within each university were randomly selected cluster samples. The survey was self-administered and included questions pertaining to menstrual symptoms, analgesic and antibiotic use patterns, and demographics. Data were analyzed using descriptive statistics and logistic regression. Results: The response rate was 95.4%. Eighty-six percent (95% CI: 83-88%) of participants experienced menstrual symptoms, and 39% (95% CI: 36-43%) reported using analgesics to treat them. Overall, 24% (95% CI: 21-27%) of participants reported self-medicated use of antibiotics to treat the following menstrual symptoms: cramps, bloating, heavy bleeding, headaches, pimples/acne, moodiness, tender breasts, backache, joint and muscle pain. Factors associated with this usage were: lower levels of education (Odds Ratio (OR): 2.8, 95% CI: 1.1-7.1, p-value: 0.03); nonscience major (OR: 1.58, 95% CI: 1.03-2.50, p-value: 0.04); usage of analgesics (OR: 3.17, 95% CI: 2.07-4.86, p-value: <0.001); and mild to extreme heavy bleeding (OR: 1.64, 95% CI: 1.01-2.67, p-value: 0.05) and pimples/acne (OR: 1.57, 95% CI: 0.98-2.54, p-value: 0.06). Ampicillin, tetracycline, ciprofloxacin and metronidazole were used to treat the most symptoms. Doctors or nurses (6%, 95% CI: 4-7%), friends (6%, 95% CI: 4-7%) and family members (7%, 95% CI: 5-8%) were most likely to recommend the use of antibiotics for menstrual symptoms, while these drugs were most often obtained from local chemists or pharmacists (10.2%, 95% CI: 8-12%). Conclusions: This is the first formal study to report that approximately 1 out of 4 university women surveyed in Southwest Nigeria self-medicate with antibiotics to treat menstrual symptoms. This practice could provide monthly, low-dose exposures to antibiotics among users. Further studies are necessary to evaluate the impacts of selfmedication on student health.
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    ISOLATION, IDENTIFICATION, AND ANTIMICROBIAL SUSCEPTIBILITY ANALYSIS OF ENTEROCOCCCUS SPP. AND SALMONELLA SPP. FROM CONVENTIONAL POULTRY FARMS TRANSITIONING TO ORGANIC FARMING PRACTICES
    (2009) Kinney, Erinna Lea; Sapkota, Amy R.; Public and Community Health; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    This baseline study evaluated prevalence and antibiotic resistance of food-borne bacteria as conventional poultry facilities transition to organic practices. Poultry litter, feed, soil, water samples and poultry questionnaire responses were collected from 10 conventional and 10 organic-transitioning poultry houses from March to June 2008. Enterococcus spp. (n=260) and Salmonella spp. (n=100) isolates were identified to species level and antimicrobial susceptibility testing was performed using the Sensititre® system. Statistical analyses were performed using STATA 10. Prevalence of Enterococcus spp. on organic-transitioning and conventional poultry farms was 100%; and prevalence of Salmonella spp. was 100% and 40%, respectively. Enterococcus isolates from conventional poultry houses displayed significantly higher percentages of resistance for 9 antibiotic agents compared to organic-transitioning isolates. Conversely, Salmonella spp. isolated from both conventional and organic-transitioning poultry houses exhibited similar antibiotic resistance patterns. Baseline findings suggest importance of poultry production practice in prevalence and antibiotic resistance patterns of food-borne bacteria.