Entomology

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Subject: search.filters.subject.Halyomorpha halys

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    Rapid transcriptome sequencing of an invasive pest, the brown marmorated stink bug Halyomorpha halys
    (Springer Nature, 2014-08-29) Ioannidis, Panagiotis; Lu, Yong; Kumar, Nikhil; Creasy, Todd; Daugherty, Sean; Chibucos, Marcus C; Orvis, Joshua; Shetty, Amol; Ott, Sandra; Flowers, Melissa; Sengamalay, Naomi; Tallon, Luke J; Pick, Leslie; Dunning Hotopp, Julie C
    Halyomorpha halys (Stål) (Insecta:Hemiptera;Pentatomidae), commonly known as the Brown Marmorated Stink Bug (BMSB), is an invasive pest of the mid-Atlantic region of the United States, causing economically important damage to a wide range of crops. Native to Asia, BMSB was first observed in Allentown, PA, USA, in 1996, and this pest is now well-established throughout the US mid-Atlantic region and beyond. In addition to the serious threat BMSB poses to agriculture, BMSB has become a nuisance to homeowners, invading home gardens and congregating in large numbers in human-made structures, including homes, to overwinter. Despite its significance as an agricultural pest with limited control options, only 100 bp of BMSB sequence data was available in public databases when this project began. Transcriptome sequencing was undertaken to provide a molecular resource to the research community to inform the development of pest control strategies and to provide molecular data for population genetics studies of BMSB. Using normalized, strand-specific libraries, we sequenced pools of all BMSB life stages on the Illumina HiSeq. Trinity was used to assemble 200,000 putative transcripts in >100,000 components. A novel bioinformatic method that analyzed the strand-specificity of the data reduced this to 53,071 putative transcripts from 18,573 components. By integrating multiple other data types, we narrowed this further to 13,211 representative transcripts. Bacterial endosymbiont genes were identified in this dataset, some of which have a copy number consistent with being lateral gene transfers between endosymbiont genomes and Hemiptera, including ankyrin-repeat related proteins, lysozyme, and mannanase. Such genes and endosymbionts may provide novel targets for BMSB-specific biocontrol. This study demonstrates the utility of strand-specific sequencing in generating shotgun transcriptomes and that rapid sequencing shotgun transcriptomes is possible without the need for extensive inbreeding to generate homozygous lines. Such sequencing can provide a rapid response to pest invasions similar to that already described for disease epidemiology.
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    PATTERNS OF HOST USE BY BROWN MARMORATED STINK BUG, HALYOMORPHA HALYS (HEMIPTERA: PENTATOMIDAE), IN WOODY ORNAMENTAL TREES AND SHRUBS
    (2015) Bergmann, Erik John; Raupp, Michael J; Entomology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Brown marmorated stink bug, Halyomorpha halys (Stål), an invasive pest species native to Asia was introduced to North America in the 1990’s. It has caused significant losses to a wide range of agricultural crops. H. halys is a nuisance pest invading homes and structures where it overwinters. I explore host use of H. halys on 254 cultivars of woody ornamental plants grown at commercial nurseries in Maryland. Overall, 88 host and 43 non-host cultivars were identified. Angiosperms supported greater abundances of H. halys than gymnosperms. Asian cultivars housed fewer H. halys than non-Asian cultivars. This trend was strongest in Acer, Ulmus, and Pyrus. Plants native to the invaded realm appear at greater risk to invasive pests than plants in the invaders aboriginal realm. Identifying cultivars most used and least used by H. halys enables growers to sell refractory cultivars making landscapes less supportive to H. halys and more sustainable.
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    An assessment of host preference, reproductive suitability and feeding injury of the brown marmorated stink bug, Halyomorpha halys, on selected vegetables
    (2014) Zobel, Emily S.; Hooks, Cerruti R.R.; Entomology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    The brown marmorated stink bug, Halyomorpha halys (Heteroptera), is an invasive insect from Asia that has become a major agricultural pest of field and vegetable crops in the Mid-Atlantic States. A field study was conducted to asses the seasonal abundance, host plant preference, reproductive suitability, and injury potential of H. haly on green bean, sweet corn, eggplant, okra and bell pepper. H. halys abundance, life stage phenology, and resulting feeding injury were monitored biweekly throughout the growing season. Overall seasonal abundance consisted of both overwintered adults and their F1 progeny. Sweet corn, okra and bell pepper had significantly higher abundances of H. halys compared to green bean, eggplant, and tomato. Eggplant, okra and bell pepper were the most suitable host plants for H. halys reproduction and development. Sweet corn, okra, bell pepper and tomato were very susceptible to feeding injury and experienced the highest injury rate per stink bug day. The implications of these findings with respect to sampling and management of H. halys in vegetable production are discussed.
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    INDIGENOUS NATURAL ENEMIES OF THE INVASIVE BROWN MARMORATED STINK BUG, HALYOMORPHA HALYS (HEMIPTERA: PENTATOMIDAE)
    (2013) Jones, Ashley Lynn; Shrewsbury, Paula M; Hooks, Cerruti RR; Entomology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Brown marmorated stink bug (BMSB), Halyomorpha halys (Stål), is an invasive species native to Southeastern Asia. Since its arrival into the U.S., BMSB has become an economically important pest in many cropping systems, including woody ornamental plants. Here I have explored the potential impact of indigenous natural enemies on BMSB in woody ornamental nursery systems in Maryland. When sampling for indigenous natural enemies in 2012 and 2013, I found seven species of egg parasitoids attacking BMSB with especially high parasitism rates from Anastatus reduvii. Overall egg mortality averaged 58% and parasitism rates increased from32% in 2012 to 44% in 2013. When sampling for predators as biological control agents, predation was low overall. I found that Arilus cristatus consumed more BMSB than any other predator species tested though low abundances were observed in the field. I also found that the use of sentinel egg masses may underestimate rates of parasitism.