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    Mechanisms of translocation of proteins through bacterial membranes
    (1988) Baykousheva, Svetla
    (Reproduced with permission from the Chemical Abstracts Service) A review with 178 refs. on translocation of proteins through bacterial membranes. The development of ideas on the mechanism of this process (the signal hypothesis, the loop model, etc.) is followed. The differences between prokaryotes and eukaryotes with respect to protein transport are discussed. Data for the involvement of biol. membranes in protein translocation are presented. The contribution of some new genetic approaches to the study of this process is summarized and the perspectives of using these methods to solve important theor. and practical problems (particularly for biotechnol.) are outlined.
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    Studies on the ultrastructure of Yersinia pseudotuberculosis after treatment with some detergents and solvents
    (Gustav Fischer Verlag, Stuttgart, 1977) Cherepova, Nadja; Baykousheva, Svetla; Veljanov, D
    The ultrastructural changes in 3 strains Yersinia pseudotuberculosis with different virulence after treatment with sodium lauryl sulfate (SLS) and petroleum ether were studied. The ultrafine sections after treatment with SLS show heavy destructive changes, concerning the cell wall, the cytoplasmic membrane and the inner structure of the cell. It was established that the same cells Y. pseudotuberculosis after cultivation on a medium with glycerol show a tendency to recover their ultrastructure. The cells treated with petroleum ether did not exhibit any notable ultrastructural changes.
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    Ultracytochemical localization of ATP-hydrolysing activity in vegetative cells, spores and isolated cytoplasmic membranes of Bacillus subtilis 168
    (1986) Cherepova, N. V.; Baykousheva, S. P.; Ilieva, K. Z.
    The localization of ATP-hydrolysing activity in vegetative cells, spores and isolated membranes of Bacillus subtilis 168 was studied by a cytochemical method combined with electron microscopy. The activity was located mainly in the cytoplasmic membrane and the mesosomes, and was also found in the inner layer of the cell wall facing the cytoplasmic membrane. Activity was also detected in the cross-membranes of dividing cells and in spore coats. The product of the reaction was observed either as fine electron-dense granules incorporated into the membranes, or as high-contrast lead precipitates on the surfaces of the membranes.