Theses and Dissertations from UMD
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Item PREVENTION AND TREATMENT OF POLYCHLORINATED BIPHENYLS IN SEDIMENTS - SOURCES AND SOLUTIONS(2019) Jing, Ran; Kjellerup, Birthe Veno; Civil Engineering; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)PCBs are classified as one of the persistent organic pollutants (POPs) with high toxicity and have undesirable effects on the environment and on humans. Once released into the environment PCBs could bioaccumulate within the food chain, due to their high affinity for organic materials. Recently, studies indicated PCBs can potentially enter a wastewater treatment plant (WWTP) system and be discharged via wastewater effluents thereby further contaminating the downstream environments. This study evaluated the potential for bioremediation of polychlorinated biphenyls (PCBs) in the effluent from a large WWTP. It was found that the continuous effluent was responsible for the majority of the discharged PCB into the receiving river (1821 g for five years), while the intermittent discharge contributed 260 g over the five years. The average number of chlorine per biphenyl for the detected PCB congeners showed a 19% difference between the two types of effluent, which indicated a potential for organohalide respiration of PCBs during the continuous treatment. This was further supported by a high level of tri-, tetra- and penta- chlorinated congeners accounting for 75% of the anaerobically respired PCBs. Potential for aerobic degradation and thus biomineralization of PCBs were identified for both effluents. In addition, the similarity of organohalide respiring (OHR) microbial populations in biosolids and intestinal human biofilms was determined by applying a bioinformatics approach. The OHR populations of the communities were analyzed from existing American and Chinese human intestinal microbiomes. The results of the biosolids analysis showed increased amounts of products from PCB respiration. Simultaneously, experiments with organohalide respiration of PCE in biosolids samples showed significant decreases in PCE concentration after 46 days (28-92%). Subsequently, it was evaluated if the OHR microbial populations in biosolids were similar to those present in intestinal human biofilms by applying a bioinformatic approach. The OHR populations of the communities were analyzed from existing American and Chinese human intestinal microbiomes. The overall groups Proteobacteria, Bacteroides, Actinobacteria, and Firmicutes phyla dominated the microbiomes in all datasets. The OHR groups in biosolids and intestinal biofilms included Dehalogenimonas, Dehalobacter, Desulfitibacter, Desulfovibrio, Sulfurospirillum, Clostridium, and Comamonas. The results of this study showed that several OHR phyla were present in all samples independent of origin. Wastewater and intestinal microbiomes also contained OHR phyla. Finally, biofilms made up by the OHR bacteria Dehalobium chlorocoercia DF-1 were inoculated on the surface of the pinewood biochar particles. The mole percent of the total PCE in the headspace decreased from 100% to 70.4%±17.6% for the rest of nine mesocosms which suggested that the D. chlorocoercia DF-1 biofilm converted PCE to TCE. The gene copy numbers of DF-1 biofilm from nine mesocosms which are ranging from 1.95×108 to 8.30×108 gene copies/g pinewood biochar. The biochar-biofilms were subsequently applied to PCB contaminated sediment from the Grass River in Michigan, USA. The goal was to evaluate the organohalide respiration of the PCB contaminated sediments in the absence/presence of the biofilm and free-floating inoculum.Item PATTERNS OF WETLAND PLANT SPECIES RICHNESS ACROSS ESTUARINE RIVER GRADIENTS(2009) Sharpe, Peter James; Baldwin, Andrew H; Marine-Estuarine-Environmental Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)It is widely accepted that in coastal wetlands a negative relationship exists between plant species richness (number of species) and salinity. However, the distribution of species richness across estuarine salinity gradients has not been closely examined. I hypothesized that plant species richness in coastal marshes (i.e., wetlands dominated by herbaceous plants) would follow a non-linear pattern with increased distance (salinity) downriver (Chapter 2). To test this hypothesis I conducted detailed marsh vegetation surveys along ≈ 50 km estuarine river gradients of the Nanticoke and Patuxent Rivers, MD/DE. I further hypothesized that the observed patterns of plant species richness on the Nanticoke and Patuxent Rivers could be accurately predicted by a mid-domain effect (MDE) model independent of measured abiotic factors using RangeModel 5.0 (Chapter 3). Lastly, I theorized that Marsh mesocosms subjected to intermediate salinity and inundation would exhibit significantly higher biomass and plant species richness compared to mesocosms subjected to extreme salt/fresh and flooding regimes utilizing a controlled greenhouse experiment (Chapter 4). I found that plant species richness can vary in both a linear (Patuxent River) and non-linear (Nanticoke River) pattern along an estuarine gradient. The MDE model did not explain a high proportion of the observed richness patterns for either river system compared to abiotic factors like porewater salinity. The controlled marsh mesocosm experiment supported the non-linear pattern of plant species richness observed along the Nanticoke River gradient, but did not show a significant difference in plant biomass or richness/diversity between purely fresh and low-salinity marsh mesocosms (α = 0.05). The results of this research suggest that tidal marsh plant richness/diversity patterns do not always conform to a simple linear relationship with increasing salinity and that the MDE is not as important of a mechanism in these communities compared to porewater salinity or flooding frequency. Furthermore tidal low salinity marshes exposed to elevated salinity and flooding frequencies are likely to see a shift in their plant community structure to more salt tolerant plants and less rich/diverse communities assuming they can accrete at a rate equal to or exceeding the present rates of sea-level rise in the Chesapeake Bay.