Theses and Dissertations from UMD

Permanent URI for this communityhttp://hdl.handle.net/1903/2

New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a give thesis/dissertation in DRUM

More information is available at Theses and Dissertations at University of Maryland Libraries.

Browse

Filters

20151

Settings

Author: search.filters.author.Zviman, Adam LyleSubject: search.filters.subject.Hypertension

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    NOVEL METHODOLOGY FOR CALCIUM MEASUREMENTS IN CONSCIOUS MICE: AN APPLICATION TO ARTERIOLAR VASOMOTION
    (2015) Zviman, Adam Lyle; Wier, Withrow G; Chen, Yu; Bioengineering; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    We developed a method employing two-photon microscopy and genetically engineered ‘Ca2+- biosensor’ mice to measure Ca2+ signaling in arteries of conscious, head-fixed mice. Arterial blood pressure was measured simultaneously via implanted telemetric pressure transducers. These methods allowed, for the first time, the study of control of arterial [Ca2+], diameter, and blood pressure by sympathetic nerve activity (SNA) as it normally exists in conscious animals. We tested the hypothesis that arterial vasomotion, observed more frequently in conscious animals than in anesthetized animals, was generated by SNA that caused synchronous Ca2+-oscillations in smooth muscle. We also measured the changes in arterial [Ca2+] that occur during the time course of experimental hypertension. Pharmacological block of SNA and isoflurane anesthesia eliminated and attenuated, respectively, vasomotion and reduced arterial [Ca2+] (320 ± 48 nM to 241 ± 20 nM and 302 ± 48 nM). This method allows for longitudinal studies of important chronic vascular pathologies.