Theses and Dissertations from UMD

Permanent URI for this communityhttp://hdl.handle.net/1903/2

New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a give thesis/dissertation in DRUM

More information is available at Theses and Dissertations at University of Maryland Libraries.

Browse

Filters

20121

Settings

Author: search.filters.author.Rogers, James AllenSubject: search.filters.subject.Molecular biology

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Developing Tools for Investigating Chemotaxis Signal Clusters in Bacillus subtilis
    (2012) Rogers, James Allen; Stewart, Richard C; Cell Biology & Molecular Genetics; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Many bacteria make use of a set of dedicated chemoreceptor proteins to control a His-Asp signaling system; this control converts environmental sensory information into instructions that regulate flagellar rotation, enabling chemotaxis. This thesis summarizes my investigations of some of the chemotaxis signaling proteins in Bacillus subtilis, particularly coupling proteins CheW and CheV. Proteins CheA, CheW, CheV, CheY, and FliM were each expressed in B. subtilis as translational fusions with either YFP or CFP. These fusion proteins were then shown to fluoresce in living bacterial cells. Motility experiments were conducted to compare the function of these fusion proteins to their wild type counterparts. This thesis proposes a series of experiments that would use these fluorescent fusion proteins to further explore the idea that these chemotaxis proteins change position when B. subtilis encounters chemostimuli.