Theses and Dissertations from UMD

Permanent URI for this communityhttp://hdl.handle.net/1903/2

New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a give thesis/dissertation in DRUM

More information is available at Theses and Dissertations at University of Maryland Libraries.

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Author: search.filters.author.Mahlandt, Alexander RobertSubject: search.filters.subject.molecular biology

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    MOLECULAR ISOLATION OF THE MOV-1 LOCUS IN HEXAPLOID BREAD WHEAT
    (2020) Mahlandt, Alexander Robert; Tiwari, Vijay K; Plant Science and Landscape Architecture (PSLA); Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    Wheat provides a substantial portion of globally consumed calories, yet faces growing challenges to meet yield needs. Traits affecting floral architecture and grain number hold immense promise for increasing yield potential. Multi-ovary wheat is a unique mutant line that holds potential in improving yields, characterized by the formation of 2-3 grains per floret in a given wheat spike. Described is a multi-approach mapping effort that refines the chromosomal position of the MOV1 locus at high resolution. A radiation hybrid map, linkage map, and refined physical map are reported. Further mutation analysis recovered missense mutations within a candidate gene, displaying a quantitatively reduced phenotype. Expression profiling of the candidate gene reveals up-regulation at crucial developmental stages. Structural investigation of the gene and surrounding locus identified two insertion/deletion events that suggest a pathway for transcriptional regulation. The results presented implicate the candidate gene in the manifestation of the multi-ovary phenotype.