Theses and Dissertations from UMD

Permanent URI for this communityhttp://hdl.handle.net/1903/2

New submissions to the thesis/dissertation collections are added automatically as they are received from the Graduate School. Currently, the Graduate School deposits all theses and dissertations from a given semester after the official graduation date. This means that there may be up to a 4 month delay in the appearance of a give thesis/dissertation in DRUM

More information is available at Theses and Dissertations at University of Maryland Libraries.

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    In Vitro Measures of MDR-Transporter Function and Whole-Hive Exposure Dynamics Using Fluorescent Dyes
    (2014) Kunkel, Grace R.; Hawthorne, David; Entomology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)
    We need to study Apis mellifera both in vivo and ex vivo to better understand honey bee biology. In vivo synergism of chemicals can occur when xenobiotic transporters are inhibited by one chemical, allowing a second chemical to accumulate and become toxic. I have conducted assays between 2010 and 2013 that demonstrated RhB dye- a xenobiotic transporter substrate, is fed in the presence of the xenobiotic inhibitor verapamil, it is found in higher levels in the hemolymph of the Apis mellifera Two types of bee food combined with two dyes were tested in 2012 for the impact of food type, and the impact of dye type on the fate of the dye in a Apis mellifera hive. Slightly hydrophobic RhB and slightly hydrophilic UrO were used. Dyed syrup persisted longer in hives than dyed pollen patties, and dyes did not spread uniformly throughout the hive.