(Springer Nature, 2019-05-17) Bertke, Michelle M.; Dubiak, Kyle M.; Cronin, Laura; Zeng, Erliang; Huber, Paul W.
Adenovirus protein, Gam1, triggers the proteolytic destruction of the E1 SUMO-activating enzyme. Microinjection of an empirically determined amount of Gam1 mRNA into one-cell Xenopus embryos can reduce SUMOylation activity to undetectable, but nonlethal, levels, enabling an examination of the role of this post-translational modification during early vertebrate development.
