Browsing by Author "Niba, Vanessa"
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Item Fabrication of Poly (D,L-Lactic-Co-Glycolic Acid) Microparticles for Improved Human Papillomavirus Vaccine Delivery(2015) Brown, Rachel; Cesewski, Ellen; Fix, Jonathan; Freudenberger, Devon; Higgins, Kara; McMahon, Eileen; Niba, Vanessa; Park, Hoon; Perdomo, Gabriela; Seo, Anna; Srivastava, Avantika; Tsui, Christina; Whiteman, Aaron; Zubajlo, Rebecca; Fisher, John P.Human papillomavirus (HPV) is the leading cause of cervical cancer and the most prevalent sexually transmitted disease worldwide. HPV vaccines require a multi-dose regimen to provide immunity, contributing to low patient compliance. We addressed this problem by formulating biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) microparticles and assessing their viability for use in controlled-release vaccines. We hypothesized that we could alter fabrication parameters to produce 1-10 μm microparticles in order to encapsulate ovalbumin (OVA) and HPV virus-like particles (VLPs). Microparticles were fabricated using a double emulsion method and used to elicit an immune response in JAWSII cells. Our results contribute to knowledge of vaccine delivery mechanisms and controlled-release technology, and could contribute to the creation of a viable controlled-release HPV vaccine.