Development of an Endoscopic Auto-Fluorescent Sensing Device to Aid in the Detection of Breast Cancer and Inform Photodynamic Therapy

Simple item page
dc.contributor.authorGaitan, Brandon
dc.contributor.authorInglut, Collin
dc.contributor.authorKanniyappan, Udayakumar
dc.contributor.authorXu, He N.
dc.contributor.authorConant, Emily F.
dc.contributor.authorFrankle, Lucas
dc.contributor.authorLi, Lin Z.
dc.contributor.authorChen, Yu
dc.contributor.authorHuang, Huang-Chiao
dc.date.accessioned2023-10-17T14:51:26Z
dc.date.available2023-10-17T14:51:26Z
dc.date.issued2022-11-11
dc.description.abstractBreast cancer is the most diagnosed cancer type in women, with it being the second most deadly cancer in terms of total yearly mortality. Due to the prevalence of this disease, better methods are needed for both detection and treatment. Reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) are autofluorescent biomarkers that lend insight into cell and tissue metabolism. As such, we developed an endoscopic device to measure these metabolites in tissue to differentiate between malignant tumors and normal tissue. We performed initial validations in liquid phantoms as well as compared to a previously validated redox imaging system. We also imaged ex vivo tissue samples after modulation with carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) and a combination of rotenone and antimycin A. We then imaged the rim and the core of MDA-MB-231 breast cancer tumors, with our results showing that the core of a cancerous lesion has a significantly higher optical redox ratio ([FAD]/([FAD] + [NADH])) than the rim, which agrees with previously published results. The mouse muscle tissues exhibited a significantly lower FAD, higher NADH, and lower redox ratio compared to the tumor core or rim. We also used the endoscope to measure NADH and FAD after photodynamic therapy treatment, a light-activated treatment methodology. Our results found that the NADH signal increases in the malignancy rim and core, while the core of cancers demonstrated a significant increase in the FAD signal.
dc.description.urihttps://doi.org/10.3390/metabo12111097
dc.identifierhttps://doi.org/10.13016/dspace/dk8l-ei0i
dc.identifier.citationGaitan, B.; Inglut, C.; Kanniyappan, U.; Xu, H.N.; Conant, E.F.; Frankle, L.; Li, L.Z.; Chen, Y.; Huang, H.-C. Development of an Endoscopic Auto-Fluorescent Sensing Device to Aid in the Detection of Breast Cancer and Inform Photodynamic Therapy. Metabolites 2022, 12, 1097.
dc.identifier.urihttp://hdl.handle.net/1903/31041
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtA. James Clark School of Engineeringen_us
dc.relation.isAvailableAtFischell Department of Bioengineeringen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectoptical redox ratio
dc.subjectbreast cancer
dc.subjectautofluorescence
dc.subjectmolecular endoscopic imaging
dc.subjectphotodynamic therapy
dc.titleDevelopment of an Endoscopic Auto-Fluorescent Sensing Device to Aid in the Detection of Breast Cancer and Inform Photodynamic Therapy
dc.typeArticle
local.equitableAccessSubmissionNo

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
metabolites-12-01097.pdf
Size:
3.32 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.55 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Fischell Department of Bioengineering Research Works