Inhibitors of Autoinducer-2 Quorum Sensing and Their Effect on Bacterial Biofilm Formation

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dc.contributor.advisorBentley, William Een_US
dc.contributor.authorLennen, Rebecca Melissaen_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2007-09-28T15:00:04Z
dc.date.available2007-09-28T15:00:04Z
dc.date.issued2007-07-31en_US
dc.description.abstractBacteria utilize small signaling molecules, or autoinducers, to regulate their gene expression in tandem by a process termed quorum sensing. The gene encoding the synthase for autoinducer-2 (AI-2), luxS, is conserved in dozens of diverse bacteria. Behaviors controlled by AI-2 include virulence, motility, toxin production, and biofilm formation. The development of therapies that interfere with AI-2 quorum sensing are attractive for targeting biofilms, which exhibit inherent resistance to most antibiotics and biocidal agents. In this study, in vitro synthesized AI-2, LuxS inhibitors, and (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone were screened for their effect on biofilm formation in Escherichia coli, Bacillus cereus, and Listeria innocua. The LuxS inhibitors were found to have no influence on biofilm formation in any of the screened species, but reduced exponential phase AI-2 production in Listeria innocua. The brominated furanone significantly inhibited growth in B. cereus and L. innocua, and under certain conditions preferentially inhibited biofilm formation independently from growth.en_US
dc.format.extent725866 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/7285
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pqcontrolledBiology, Microbiologyen_US
dc.subject.pquncontrolledquorum sensingen_US
dc.subject.pquncontrolledautoinducer-2en_US
dc.subject.pquncontrolledbiofilmen_US
dc.subject.pquncontrolledquorum sensing inhibitorsen_US
dc.subject.pquncontrolledbrominated furanoneen_US
dc.titleInhibitors of Autoinducer-2 Quorum Sensing and Their Effect on Bacterial Biofilm Formationen_US
dc.typeThesisen_US

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