ROLE OF SELECTED EXTRACELLULAR FUNCTION SIGMA FACTORS IN CARBOHYDRASE GENE EXPRESSION IN SACCHAROPHAGUS DEGRADANS 2-40

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dc.contributor.advisorHutcheson, Stevenen_US
dc.contributor.authorSinnott, Corinnen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2011-10-08T06:40:06Z
dc.date.available2011-10-08T06:40:06Z
dc.date.issued2011en_US
dc.description.abstractThis project explores the role of selected ECF sigma factors in regulating carbohydrase gene expression in Saccharophagus degradans 2-40. ECF sigma factors most likely to be involved in carbohydrase regulation were identified by scoring for the presence of high sequence similarity in cellulolytic but not in non-cellulolytic bacterial species. Cellvibrio japonicus, Teredinibacter turnerae, and Hahella chejuensis were determined to be the cellulolytic species most appropriate for evaluating transcriptional regulators for a role in carbohydrase regulation. Two sigma factors were identified in this survey. These sigma factor genes were cloned into a plasmid vector and expressed in S. degradans. Cellulase activity was detected for cell lysates of glucose-grown cultures of a strain expressing the sigma factor corresponding to gene 2407. It was concluded that sigma factor 2407 is involved in the regulation of endoglucanases in the S. degradans cellulolytic system.en_US
dc.identifier.urihttp://hdl.handle.net/1903/12085
dc.subject.pqcontrolledGeneticsen_US
dc.titleROLE OF SELECTED EXTRACELLULAR FUNCTION SIGMA FACTORS IN CARBOHYDRASE GENE EXPRESSION IN SACCHAROPHAGUS DEGRADANS 2-40en_US
dc.typeThesisen_US

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