Effects of chronic exercise on global DNA methylation and epigenetic factors in sperm and testes of mice.

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dc.contributor.advisorRoth, Stephen Men_US
dc.contributor.authorMarini, Michael Paulen_US
dc.contributor.departmentKinesiologyen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2013-07-02T05:30:06Z
dc.date.available2013-07-02T05:30:06Z
dc.date.issued2012en_US
dc.description.abstractEpigenetic alterations of DNA affect DNA transcription and translation. These alterations occur frequently, however environmental exposures induce epigenetic changes to DNA that would otherwise remain in autoregulatory stasis. This study aimed to look at exercise as a possible environmental factor causing epigenetic change. The study also assessed global DNA methylation in sperm, which may transmit such epigenetic changes via the paternal germ line. Measurements were compared between groups of mice that engaged in chronic exercise or remained sedentary. This study also examined enzymes causing methylation shifts in sperm by comparing levels of mRNA expression of genes responsible for new DNA methylation - DNMT3A, DNMT3B and DNMT3L - in testes. These results were compared between exercise and sedentary cohorts, and in progeny to assess heritability of epigenetic change. The results showed a significant difference in global methylation in the sperm between exercise and sedentary cohorts and a concomitant increase in gene expression in multiple DNMT3 genes.en_US
dc.identifier.urihttp://hdl.handle.net/1903/14194
dc.subject.pqcontrolledKinesiologyen_US
dc.subject.pquncontrolledDNAen_US
dc.subject.pquncontrolledExerciseen_US
dc.subject.pquncontrolledIn-uteroen_US
dc.subject.pquncontrolledMethylationen_US
dc.subject.pquncontrolledSpermen_US
dc.subject.pquncontrolledTestesen_US
dc.titleEffects of chronic exercise on global DNA methylation and epigenetic factors in sperm and testes of mice.en_US
dc.typeThesisen_US

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