TIME SERIES TRANSCRIPTIONAL PROFILING ANALYSIS OF THE Arabidopsis thaliana USING FULL GENOME DNA MICROARRAY AND METABOLIC INFORMATION

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dc.contributor.advisorKlapa, Maria Ien_US
dc.contributor.advisorQuackenbush, Johnen_US
dc.contributor.authorDutta, Bhaskaren_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2004-08-27T05:41:47Z
dc.date.available2004-08-27T05:41:47Z
dc.date.issued2004-08-26en_US
dc.description.abstractWith the advent of the DNA microarray technology, it became possible to study the expression of entire cellular genomes. Tanscriptional profiling alone can not provide a comprehensive picture of the cellular physiological state and it should be complemented by other cellular fingerprints. Transcriptional profiling combined with metabolic information of a systematically perturbed system can unravel the relationship between gene and metabolic regulation. In this context the transcriptional response of Arabidopsis thaliana liquid cultures (grown for 12 days under light and 23 sup oC) to 1-day treatment with 1% CO sub 2 was measured by full genome cDNA microarrays. The Time series gene expression profiles were analyzed in the context of the known Arabidopsis thaliana physiology using multivariate statistics. Data analysis revealed an increase in the rate of CO sub 2 fixation, biomass production and cell wall growth. The breadth of the information obtained from a single experiment validated the significance of the high throughput transcriptional profiling.en_US
dc.format.extent1102251 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/1853
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pqcontrolledBiology, Plant Physiologyen_US
dc.subject.pqcontrolledBiology, Geneticsen_US
dc.subject.pquncontrolledgenome-wide transcriptional profilingen_US
dc.subject.pquncontrolledtime series analysisen_US
dc.subject.pquncontrolledDNA microarrayen_US
dc.subject.pquncontrolledArabidopsis thalianaen_US
dc.subject.pquncontrolledelevated CO2en_US
dc.subject.pquncontrolledfunctional genomicsen_US
dc.titleTIME SERIES TRANSCRIPTIONAL PROFILING ANALYSIS OF THE Arabidopsis thaliana USING FULL GENOME DNA MICROARRAY AND METABOLIC INFORMATIONen_US
dc.typeThesisen_US

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