Defining Critical Parameters for Producing and Modulating Inflammation Caused by Cell Encapsulating Alginate Microspheres

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dc.contributor.advisorWang, Nam Sunen_US
dc.contributor.advisorLyle, Dan Ben_US
dc.contributor.authorBreger, Joyce Catherineen_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2008-04-22T16:01:21Z
dc.date.available2008-04-22T16:01:21Z
dc.date.issued2007-09-11en_US
dc.description.abstractMinimizing induced inflammation, particularly nitric oxide (NO) production, is critical to optimal function or failure of implanted encapsulated cells. The purpose of this study is to define critical factors that affect toxic NO production from the macrophage cell line RAW264.7 in response to alginate microcapsules. The effects of the following were determined: 1) concentration of endotoxin (LPS) contamination; 2) presence of interferon-gamma (IFN-γ); 3) bead diameter and alginate volume; and 4) anti-inflammatory drugs in the alginate. A higher concentration (5 X) of LPS was required in alginate to produce the effect seen by LPS free in medium, sensitivity was enhanced by IFN-γ, bead diameter was inversely proportional to NO2 under low inflammatory conditions, and parthenolide in alginate significantly reduced inflammation. These results suggest that survival of implanted encapsulated cells may be improved by using highly purified alginate, avoiding ancillary inflammation, controlling surface area presentation, and incorporating anti-inflammatory drugs into the capsule matrix.en_US
dc.format.extent2036200 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/7596
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pqcontrolledBiology, Generalen_US
dc.subject.pqcontrolledEngineering, Chemicalen_US
dc.subject.pquncontrolledbiomaterialsen_US
dc.subject.pquncontrollednitric oxideen_US
dc.subject.pquncontrolledinflammationen_US
dc.subject.pquncontrolledmacrophageen_US
dc.subject.pquncontrolledalginateen_US
dc.titleDefining Critical Parameters for Producing and Modulating Inflammation Caused by Cell Encapsulating Alginate Microspheresen_US
dc.typeThesisen_US

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