Studies on the formation, composition and detection of biofilms of food-borne pathogens

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dc.contributor.advisorWeiner, Ronald Men_US
dc.contributor.authorEriksson de Rezende, Christian Luizen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2005-02-02T06:49:25Z
dc.date.available2005-02-02T06:49:25Z
dc.date.issued2004-12-06en_US
dc.description.abstractBiofilms enable food-borne pathogens to resist removal from surfaces and survive disinfection. Biofilms of pathogens [e.g., S. enterica Serovar Typhimurium DT104 (STDT104)] formed on various surface types were probed with Calcofluor (b-D-glucan-specific ) and lectins. All biofilms were detected after exposure to Calcofluor. Lectins bound to specific carbohydrates in bacterial exopolymeric substances (EPS). Results supported the in vitro use of Calcofluor and lectins as non-specific and genera-specific probes of biofilms. Next, the capsular polysaccharide (CP) of STDT104 was extracted and shown to contain glucose, mannose and trace amounts of galactose. Polyclonal antibodies against this extract were specific for a CP as demonstrated by western blots and immunoelectron microscopy. Confocal microscopy images revealed the following: thicker biofilms formed at 25?C, CP synthesis was not temperature-dependent and not produced by all cells. Synthesis of CP by STDT104 may represent an aggregative and protective substance in addition to curli and celluloseen_US
dc.format.extent7559923 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/2122
dc.language.isoen_US
dc.subject.pqcontrolledBiology, Microbiologyen_US
dc.titleStudies on the formation, composition and detection of biofilms of food-borne pathogensen_US
dc.typeThesisen_US

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